Rabbit anti-Human Ob-R Polyclonal Antibody | anti-Ob-R antibody

Ob-R (L662) Polyclonal Antibody

Cat. Num. AAA3003769

• Gene Names: LEPR; OBR; OB-R; CD295; LEP-R; LEPRD
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Ob-R; Polyclonal Antibody; Ob-R (L662) Polyclonal Antibody; Leptin receptor; LEP-R; HuB219 OB receptor; OB-R; CD295; LEPR; DB; OBR; anti-Ob-R antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Ob-R (L662) polyclonal antibody detects endogenous levels of Ob-R protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 896

• Applicable Applications for anti-Ob-R antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 650-700 of Human Ob-R.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Ob-R (L662) pAb at 1:500 dilutionLane1:PC3 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:SK-OVCAR3 whole cell lysate (40ug) Lane4:L02 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Ob-R (L662) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Related Product Information for anti-Ob-R antibody

The first such recessive obesity mutation, the obese mutation (Ob), was identified in 1950. Mutation of Ob results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. It has been postulated that the Ob gene product may function as a component of a signaling pathway in adipose tissue that functions to regulate body fat depot size. The cloning and sequence analysis of the mouse Ob gene and its human homolog has recently been described. Ob encodes an adipose tissue-specific mRNA with a highly conserved 167 amino acid open reading frame. The predicted amino acid sequence is 84% identical between human and mouse and has the features of a secreted protein. A nonsense mutation in codon 105 has been found in the original congenic C57BL/6J Ob/Ob mouse strain. The Ob gene encodes the protein leptin.

NCBI and Uniprot Product Information

• NCBI GI #: 51093379
• NCBI GeneID: 3953
• NCBI Accession #: NP_001003679.1
• NCBI GenBank Nucleotide #: NP_001003679.1
• UniProt Accession #: P48357
• Molecular Weight: ~ 132kDa
• NCBI Official Full Name: leptin receptor isoform 3
• NCBI Official Synonym Full Names: leptin receptor
• NCBI Official Symbol: LEPR
• NCBI Official Synonym Symbols: OBR; OB-R; CD295; LEP-R; LEPRD
• NCBI Protein Information: leptin receptor
• UniProt Protein Name: Leptin receptor
• UniProt Gene Name: LEPR
• UniProt Synonym Gene Names: DB; OBR; LEP-R; OB-R
• UniProt Entry Name: LEPR_HUMAN

NCBI Description

The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010]

Uniprot Description

LEPR: the receptor for leptin (obesity factor). A single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Five alternatively spliced transcript variants have been reported.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: integral to membrane; plasma membrane; extracellular region; receptor complex

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; identical protein binding; protein binding; transmembrane receptor activity

Biological Process: cholesterol metabolic process; cell surface receptor linked signal transduction; negative regulation of hydrolase activity; multicellular organismal development; negative regulation of gluconeogenesis; cytokine and chemokine mediated signaling pathway; energy reserve metabolic process

Disease: Leptin Receptor Deficiency