Rabbit anti-Human MGMT Polyclonal Antibody | anti-MGMT antibody

MGMT, CT (O-6-Methylguanine DNA Methyltransferase, 6-O-Methylguanine DNA Methyltransferase, Methylated DNA Protein Cysteine Methyltransferase, Methylguanine DNA Methyltransferase, O-6-Methylguanine DNA Alkyltransferase) (FITC)

Cat. Num. AAA6488056

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: MGMT; Polyclonal Antibody; CT (O-6-Methylguanine DNA Methyltransferase; 6-O-Methylguanine DNA Methyltransferase; Methylated DNA Protein Cysteine Methyltransferase; Methylguanine DNA Methyltransferase; O-6-Methylguanine DNA Alkyltransferase) (FITC); EC=2.1.1.63; anti-MGMT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MGMT.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MGMT antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human MGMT (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of MGMT antibody (C-term) in CEM cell line lysates (35ug/lane). MGMT (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human placenta with MGMT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of CEM cells using MGMT Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of MGMT (arrow) using rabbit polyclonal MGMT Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MGMT gene.)

Product Categories/Family for anti-MGMT antibody

Antibodies; Enzymes; Methyltransferase

References

1. Kim, J.I., Suh, J.T. Hum. Pathol. 40(7), 934-941 (2009). 2. Chen, S.P., Chiu, S.C. Genet Test Mol Biomarkers 13(1), 67-71 (2009). 3. Slupphaug, G., Lettrem, I. Carcinogenesis 13(10), 1769-1773 (1992). 4. Lee, S.M., Crowther, D. Br. J. Cancer 66(2), 331-336 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 197304670
• NCBI GeneID: 4255
• NCBI Accession #: NP_002403
• NCBI GenBank Nucleotide #: NM_002412.4
• UniProt Accession #: P16455; Q5VY78
• Molecular Weight: 23.8 kDa (227 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: methylated-DNA--protein-cysteine methyltransferase
• NCBI Official Synonym Full Names: O-6-methylguanine-DNA methyltransferase
• NCBI Official Symbol: MGMT
• NCBI Protein Information: methylated-DNA--protein-cysteine methyltransferase
• UniProt Protein Name: Methylated-DNA--protein-cysteine methyltransferase
• Protein Family: Probable methylated-DNA--protein-cysteine methyltransferase
• UniProt Gene Name: MGMT
• UniProt Synonym Gene Names: MGMT
• UniProt Entry Name: MGMT_HUMAN

NCBI Description

Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015]

Uniprot Description

MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family.

Protein type: EC 2.1.1.63; Methyltransferase; Nuclear receptor co-regulator; DNA repair, damage; Apoptosis

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: nucleoplasm; membrane; nucleus

Molecular Function: methyltransferase activity; DNA binding; methylated-DNA-[protein]-cysteine S-methyltransferase activity; damaged DNA binding; calcium ion binding; DNA-methyltransferase activity

Biological Process: response to drug; response to ethanol; positive regulation of DNA repair; DNA dealkylation; response to folic acid; response to toxin; DNA methylation; regulation of caspase activity; DNA repair; DNA ligation