Leptin Receptor/LEPR/CD295 Recombinant Protein | LEPR recombinant protein

Recombinant Human Leptin Receptor/LEPR/CD295 Protein (C-His)

Cat. Num. AAA2557215

• Gene Names: LEPR; OBR; OB-R; CD295; LEP-R; LEPRD
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Leptin Receptor/LEPR/CD295; Recombinant Human Leptin Receptor/LEPR/CD295 Protein (C-His); Leptin receptor; LEP-R; HuB219; OB receptor; OB-R; CD295; LEPR; DB; OBR; LEPR recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH7.4.
• Sequence: Phe22-Asp839
• Sequence Length: 896

• Species: Human
• Endotoxin: < 1.0 EU per mug as determined by the LAL method.
• Fusion Tag: C-His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for LEPR recombinant protein

Protein Construction: Recombinant Human Leptin Receptor is produced by our mammalian expression system and the target gene encoding Phe22-Asp839 is expressed with a 10His tag at the C-terminus.

Background: The Leptin receptor is a member of the Class I cytokine receptor family. It mediates the activities of Leptin, a multi-functional hormone produced primarily by adipose tissues that plays roles in food intake, energy metabolism, angiogenesis, reproduction, hematopoiesis, bone metabolism, and immune function. The human Leptin R gene encodes 1165 amino acids (aa) including a signal peptide, an extracellular region with cytokine receptor homology (CRH), multiple fibronectin type III domains and a WSXWS motif, a transmembrane domain, and a cytoplasmic domain that supports JAK/STAT signaling. Soluble Leptin R is the primary Leptin-binding protein in blood, where it maintains a pool of available bioactive Leptin, delays Leptin clearance from circulation, and down-regulates blood-brain transmission of Leptin. In humans, soluble Leptin R levels are inversely proportional to adiposity and are elevated in females versus males. Soluble Leptin R is also found up-regulated in patients with chronic heart failure, end-stage renal disease, and anorexia.It is expressed by tumor-initiating stem cells, and is proposed as a link between cancer and obesity.

NCBI and Uniprot Product Information

• NCBI GI #: 51093379
• NCBI GeneID: 3953
• NCBI Accession #: NP_001003679.1
• NCBI GenBank Nucleotide #: NP_001003679.1
• UniProt Accession #: P48357
• Molecular Weight: Molecular Mass: 94.9kDa; Actual Protein Molecular Mass: 100-130kDa
• NCBI Official Full Name: leptin receptor isoform 3
• NCBI Official Synonym Full Names: leptin receptor
• NCBI Official Symbol: LEPR
• NCBI Official Synonym Symbols: OBR; OB-R; CD295; LEP-R; LEPRD
• NCBI Protein Information: leptin receptor
• UniProt Protein Name: Leptin receptor
• Protein Family: Leptin receptor
• UniProt Gene Name: LEPR
• UniProt Synonym Gene Names: DB; OBR; LEP-R; OB-R
• UniProt Entry Name: LEPR_HUMAN

NCBI Description

The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010]

Uniprot Description

LEPR: the receptor for leptin (obesity factor). A single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Five alternatively spliced transcript variants have been reported.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: integral to membrane; plasma membrane; extracellular region; receptor complex

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; identical protein binding; protein binding; transmembrane receptor activity

Biological Process: cholesterol metabolic process; cell surface receptor linked signal transduction; negative regulation of hydrolase activity; multicellular organismal development; negative regulation of gluconeogenesis; cytokine and chemokine mediated signaling pathway; energy reserve metabolic process

Disease: Leptin Receptor Deficiency