Rabbit anti-Human OASL Polyclonal Antibody | anti-Oasl2 antibody

OASL, CT (59kD 2'-5'-oligoadenylate Synthase-like Protein, p59 OASL, p59OASL, Thyroid Receptor-interacting Protein 14, TR-interacting Protein 14, TRIP-14, TRIP14)

Cat. Num. AAA630945

• Gene Names: Oasl2; Oasl; M1204; Mmu-OASL
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: OASL; Polyclonal Antibody; CT (59kD 2'-5'-oligoadenylate Synthase-like Protein; p59 OASL; p59OASL; Thyroid Receptor-interacting Protein 14; TR-interacting Protein 14; TRIP-14; TRIP14); Anti -OASL; anti-Oasl2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human OASL.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-Oasl2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human OASL (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-OASL C-term Antibody is used in Western blot to detect OASL in HL60 lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-Oasl2 antibody

2-prime,5-prime oligoadenylates (2-5As) bind to and activate RNase L, leading to degradation of RNA and inhibition of protein synthesis. 2-5As are produced by 2-5A synthetases (OASs), a highly-conserved family of interferon-induced enzymes. The predicted 514aa human p59OASL (2-5A synthetases-like) protein shares a highly conserved N-terminal domain with other OASs. The C-terminal portion of p59OASL contains 2 ubiquitin-like domains. p59OASL is expressed in most tissues, with the highest levels in hematopoietic tissues, colon, and stomach.

Product Categories/Family for anti-Oasl2 antibody

Antibodies; Abs to Enzymes; Synthase

NCBI and Uniprot Product Information

• NCBI GI #: 21707766
• NCBI GeneID: 23962
• UniProt Accession #: Q9Z2F2; Q9D6S2
• Molecular Weight: 58,767 Da
• NCBI Official Full Name: Oasl2 protein
• NCBI Official Synonym Full Names: 2'-5' oligoadenylate synthetase-like 2
• NCBI Official Symbol: Oasl2
• NCBI Official Synonym Symbols: Oasl; M1204; Mmu-OASL
• NCBI Protein Information: 2'-5'-oligoadenylate synthase-like protein 2; p54OASL; p54 OASL; 54 kDa 2'-5'-oligoadenylate synthase-like protein 2; 54 kDa 2'-5'-oligoadenylate synthetase-like protein 2
• UniProt Protein Name: 2'-5'-oligoadenylate synthase-like protein 2
• Protein Family: 2'-5'-oligoadenylate synthase-like protein
• UniProt Gene Name: Oasl2
• UniProt Synonym Gene Names: p54 OASL
• UniProt Entry Name: OASL2_MOUSE

Uniprot Description

Oasl2: Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. Synthesizes oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. Belongs to the 2-5A synthase family.

Protein type: EC 2.7.7.84

Molecular Function: transferase activity; RNA binding; metal ion binding; double-stranded RNA binding; nucleotide binding; ATP binding; 2'-5'-oligoadenylate synthetase activity; nucleotidyltransferase activity

Biological Process: immune system process; response to virus; innate immune response; immune response; purine nucleotide biosynthetic process; defense response to virus