Rabbit anti-Human OASL Polyclonal Antibody | anti-OASL antibody

OASL, CT (59kD 2'-5'-oligoadenylate Synthase-like Protein, p59 OASL, p59OASL, Thyroid Receptor-interacting Protein 14, TR-interacting Protein 14, TRIP-14, TRIP14) (Azide free) (HRP)

Cat. Num. AAA6490929

• Gene Names: OASL; OASLd; TRIP14; TRIP-14; p59OASL; p59 OASL; p59-OASL
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: OASL; Polyclonal Antibody; CT (59kD 2'-5'-oligoadenylate Synthase-like Protein; p59 OASL; p59OASL; Thyroid Receptor-interacting Protein 14; TR-interacting Protein 14; TRIP-14; TRIP14) (Azide free) (HRP); anti-OASL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human OASL.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-OASL antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human OASL (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-OASL C-term Antibody is used in Western blot to detect OASL in HL60 lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-OASL antibody

Antibodies; Enzymes; Synthase

References

1. Hovnanian, A., et al., Genomics 56(3):362-363 (1999). 2. Rebouillat, D., et al., Eur. J. Biochem. 257(2):319-330 (1998). 3. Hartmann, R., et al., Nucleic Acids Res. 26(18):4121-4128 (1998). 4. Lee, J.W., et al., Mol. Endocrinol. 9(2):243-254 (1995). 5. Mackay, V., et al., J. Biol. Chem. 251(12):3716-3719 (1976). 6. Mariko Ishibashi, et al. "Biochemical and Biophysical Research Communications. (E-pub).

NCBI and Uniprot Product Information

• NCBI GI #: 6226835
• NCBI GeneID: 8638
• UniProt Accession #: Q15646; O75686; Q17R95; Q9Y6K6; Q9Y6K7; B2RAZ2; I1YDD2
• Molecular Weight: 514
• NCBI Official Full Name: 2'-5'-oligoadenylate synthase-like protein
• NCBI Official Synonym Full Names: 2'-5'-oligoadenylate synthetase-like
• NCBI Official Symbol: OASL
• NCBI Official Synonym Symbols: OASLd; TRIP14; TRIP-14; p59OASL; p59 OASL; p59-OASL
• NCBI Protein Information: 2'-5'-oligoadenylate synthase-like protein; 2'-5'-OAS-RP; 2'-5'-OAS-related protein; TR-interacting protein 14; thyroid receptor-interacting protein 14; 59 kDa 2'-5'-oligoadenylate synthase-like protein; 59 kDa 2'-5'-oligoadenylate synthetase-like protein
• UniProt Protein Name: 2'-5'-oligoadenylate synthase-like protein
• Protein Family: 2'-5'-oligoadenylate synthase-like protein
• UniProt Gene Name: OASL
• UniProt Synonym Gene Names: TRIP14; 2'-5'-OAS-RP; TR-interacting protein 14; TRIP-14; p59OASL
• UniProt Entry Name: OASL_HUMAN

Uniprot Description

OASL: a protein of the 2'-5'-oligoadenylate synthetase family, but does not have 2'-5'-OAS activity. Binds double-stranded RNA and DNA. Interacts with the ligand binding domain of the thyroid receptor (TR). Does not require the presence of thyroid hormone for its interaction. Contains a ubiquitin-like domain that interacts with methyl CpG-binding protein 1.

Protein type: Nucleolus; DNA-binding

Chromosomal Location of Human Ortholog: 12q24.2

Cellular Component: membrane; cytoplasm; nucleolus; cytosol

Molecular Function: DNA binding; double-stranded RNA binding; thyroid hormone receptor binding; ATP binding; 2'-5'-oligoadenylate synthetase activity

Biological Process: negative regulation of viral genome replication; metabolic process; response to virus; cytokine and chemokine mediated signaling pathway; defense response to virus