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Western Blot (WB)
(Western blot analysis of extracts from COLO cells using NXPH4 antibody.)
Rabbit NXPH4 Polyclonal Antibody | anti-NXPH4 antibody
NXPH4 Antibody
Gene Names
NXPH4; NPH4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NXPH4; Polyclonal Antibody; NXPH4 Antibody; Neurexophilin 4; NPH4; NXPH 4; anti-NXPH4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
NXPH4 antibody detects endogenous levels of total NXPH4
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
308
Applicable Applications for anti-NXPH4 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Secreted
Tissue Specificity
Expressed in brain, spleen, and testis.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from COLO cells using NXPH4 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from COLO cells using NXPH4 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from hepg2, using NXPH4 Antibody. Lane 1 was treated with the blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from hepg2, using NXPH4 Antibody. Lane 1 was treated with the blocking peptide.)
Immunofluorescence (IF)
(MBS9605686 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9605686 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunohistochemistry (IHC)
(MBS9605686 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9605686 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-NXPH4 antibody
Function: May be signaling molecules that resemble neuropeptides and that act by binding to alpha-neurexins and possibly other receptors.
Post-translational Modifications: May be proteolytically processed at the boundary between the N-terminal non-conserved and the central conserved domain in neuron-like cells.
Similarity: Belongs to the neurexophilin family.
Post-translational Modifications: May be proteolytically processed at the boundary between the N-terminal non-conserved and the central conserved domain in neuron-like cells.
Similarity: Belongs to the neurexophilin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 33 kD
Predicted: 34 kDa
Predicted: 34 kDa
NCBI Official Full Name
neurexophilin-4
NCBI Official Synonym Full Names
neurexophilin 4
NCBI Official Symbol
NXPH4
NCBI Official Synonym Symbols
NPH4
NCBI Protein Information
neurexophilin-4
UniProt Protein Name
Neurexophilin-4
Protein Family
UniProt Gene Name
NXPH4
UniProt Synonym Gene Names
NPH4
Uniprot Description
May be signaling molecules that resemble neuropeptides and that act by binding to alpha-neurexins and possibly other receptors.
Similar Products
Product Notes
The NXPH4 nxph4 (Catalog #AAA9605686) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NXPH4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NXPH4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IHC: 1:50-1:200 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the NXPH4 nxph4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NXPH4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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