Mouse anti-Human UBTF Monoclonal Antibody | anti-UBTF antibody

UBTF (Nucleolar Transcription Factor 1, Upstream-binding Factor 1, UBF-1, Autoantigen NOR-90, UBF1, UBF) (HRP)

Cat. Num. AAA6155656

• Gene Names: UBTF; UBF; UBF1; UBF2; UBF-1; CONDBA; NOR-90
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: UBTF; Monoclonal Antibody; UBTF (Nucleolar Transcription Factor 1; Upstream-binding Factor 1; UBF-1; Autoantigen NOR-90; UBF1; UBF) (HRP); anti-UBTF antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 6B6
• Specificity: Recognizes human UBTF.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-UBTF antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 3ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa551-650 from human UBTF (NP_055048) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: PPAATNSSKKMKFQGEPKKPPMNGYQKFSQELLSNGELNHLPLKERMVEIGSRWQRISQSQKEHYKKLAEEQQKQYKVHLDLWVKSLSPQDRAAYKEYIS
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(UBTF monoclonal antibody, Western Blot analysis of UBTF expression in HeLa NE.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to UBTF on HeLa cell. [antibody concentration 10ug/ml])

Testing Data

(Detection limit for recombinant GST tagged UBTF is ~0.03ng/ml as a capture antibody.)

Product Categories/Family for anti-UBTF antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 7657671
• NCBI GeneID: 7343
• NCBI Accession #: NP_055048
• NCBI GenBank Nucleotide #: NM_014233
• UniProt Accession #: P17480
• Molecular Weight: 89kDa
• NCBI Official Full Name: nucleolar transcription factor 1 isoform a
• NCBI Official Synonym Full Names: upstream binding transcription factor
• NCBI Official Symbol: UBTF
• NCBI Official Synonym Symbols: UBF; UBF1; UBF2; UBF-1; CONDBA; NOR-90
• NCBI Protein Information: nucleolar transcription factor 1
• UniProt Protein Name: Nucleolar transcription factor 1
• Protein Family: Nucleolar transcription factor
• UniProt Gene Name: UBTF
• UniProt Synonym Gene Names: UBF; UBF1; UBF-1
• UniProt Entry Name: UBF1_HUMAN

NCBI Description

This gene encodes a member of the HMG-box DNA-binding protein family. The encoded protein plays a critical role in ribosomal RNA transcription as a key component of the pre-initiation complex, mediating the recruitment of RNA polymerase I to rDNA promoter regions. The encoded protein may also play important roles in chromatin remodeling and pre-rRNA processing, and its activity is regulated by both phosphorylation and acetylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3, 11 and X and the long arm of chromosome 11. [provided by RefSeq, Aug 2011]

Uniprot Description

UBF: a transcription factor that recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the species-specific factor SL1. Activate RNA polymerase II-regulated, beta-catenin-responsive promoters. Interacts with TAF1, a factor involved in the transcription of cell cycle and growth regulatory genes. It binds specifically to the upstream control element. Two splice variant isoforms have been described.

Protein type: Nucleolus; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: nucleoplasm; nucleolus

Molecular Function: protein binding; DNA binding; chromatin binding

Biological Process: chromatin remodeling; regulation of transcription from RNA polymerase I promoter; regulation of transcription, DNA-dependent; negative regulation of gene expression, epigenetic; transcription from RNA polymerase I promoter; RNA elongation from RNA polymerase I promoter; gene expression; transcription initiation from RNA polymerase I promoter; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; positive regulation of transcription from RNA polymerase I promoter