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Western Blot (WB) (NUSAP Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the NUSAP antibody detected the NUSAP protein (arrow).)

Rabbit anti-Human NUSAP Polyclonal Antibody | anti-NUSAP antibody

NUSAP, NT (NUSAP1, ANKT, Nucleolar and spindle-associated protein 1) (APC)

Gene Names
NUSAP1; LNP; ANKT; SAPL; BM037; NUSAP; Q0310; PRO0310p1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
NUSAP; Polyclonal Antibody; NT (NUSAP1; ANKT; Nucleolar and spindle-associated protein 1) (APC); NUSAP1; Nucleolar and spindle-associated protein 1; anti-NUSAP antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-NUSAP antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-100
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
NUSAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-56 amino acids from the N-terminal region of human NUSAP.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NUSAP Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the NUSAP antibody detected the NUSAP protein (arrow).)

Western Blot (WB) (NUSAP Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the NUSAP antibody detected the NUSAP protein (arrow).)

Immunohistochemistry (IHC)

(NUSAP antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NUSAP antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (NUSAP antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NUSAP antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-NUSAP antibody
NUSAP1 is a nucleolar-spindle-associated protein that plays a role in spindle microtubule organization (Raemaekers et al., 2003 [PubMed 12963707]).
Product Categories/Family for anti-NUSAP antibody
References
Wadia, P.P., et al. Blood 115(10):2077-2087(2010). Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006). Raemaekers, T., et al. J. Cell Biol. 162(6):1017-1029(2003).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
Molecular Weight
42,352 Da
NCBI Official Full Name
nucleolar and spindle-associated protein 1 isoform 1
NCBI Official Synonym Full Names
nucleolar and spindle associated protein 1
NCBI Official Symbol
NUSAP1
NCBI Official Synonym Symbols
LNP; ANKT; SAPL; BM037; NUSAP; Q0310; PRO0310p1
NCBI Protein Information
nucleolar and spindle-associated protein 1
UniProt Protein Name
Nucleolar and spindle-associated protein 1
UniProt Gene Name
NUSAP1
UniProt Synonym Gene Names
ANKT; NuSAP
UniProt Entry Name
NUSAP_HUMAN

NCBI Description

NUSAP1 is a nucleolar-spindle-associated protein that plays a role in spindle microtubule organization (Raemaekers et al., 2003 [PubMed 12963707]).[supplied by OMIM, Jun 2009]

Uniprot Description

NUSAP1: Microtubule-associated protein with the capacity to bundle and stabilize microtubules. May associate with chromosomes and promote the organization of mitotic spindle microtubules around them. Belongs to the NUSAP family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Cell cycle regulation

Chromosomal Location of Human Ortholog: 15q15.1

Cellular Component: spindle microtubule; cytoplasm; nucleolus; chromosome; nucleus

Molecular Function: DNA binding; microtubule binding

Biological Process: establishment of mitotic spindle localization; cytokinesis after mitosis; mitotic sister chromatid segregation; positive regulation of mitosis; mitotic chromosome condensation

Research Articles on NUSAP

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Product Notes

The NUSAP nusap1 (Catalog #AAA6327327) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NUSAP, NT (NUSAP1, ANKT, Nucleolar and spindle-associated protein 1) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NUSAP can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-100 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NUSAP nusap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUSAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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