Human Nucleolar and spindle-associated protein 1 ELISA Kit | NUSAP1 elisa kit

Human Nucleolar and spindle-associated protein 1 ELISA Kit

Cat. Num. AAA761588

• Gene Names: NUSAP1; LNP; ANKT; SAPL; BM037; NUSAP; Q0310; PRO0310p1
• Reactivity: Human
• Synonyms: Nucleolar and spindle-associated protein 1; Human Nucleolar and spindle-associated protein 1 ELISA Kit; NUSAP1/NuSAP/BM-037/PRO0310/ANKT/Nucleolar and spindle-associated protein 1; NUSAP1 elisa kit

• Reactivity: Human
• Sequence Length: 441

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for NUSAP1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-NUSAP1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-NUSAP1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NUSAP1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NUSAP1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 193804865
• NCBI GeneID: 51203
• NCBI Accession #: NP_057443.2
• NCBI GenBank Nucleotide #: NM_016359.4
• Molecular Weight: 42,352 Da
• NCBI Official Full Name: nucleolar and spindle-associated protein 1 isoform 1
• NCBI Official Synonym Full Names: nucleolar and spindle associated protein 1
• NCBI Official Symbol: NUSAP1
• NCBI Official Synonym Symbols: LNP; ANKT; SAPL; BM037; NUSAP; Q0310; PRO0310p1
• NCBI Protein Information: nucleolar and spindle-associated protein 1
• UniProt Protein Name: Nucleolar and spindle-associated protein 1
• Protein Family: Nucleolar and spindle-associated protein
• UniProt Gene Name: NUSAP1
• UniProt Synonym Gene Names: ANKT; NuSAP
• UniProt Entry Name: NUSAP_HUMAN

NCBI Description

NUSAP1 is a nucleolar-spindle-associated protein that plays a role in spindle microtubule organization (Raemaekers et al., 2003 [PubMed 12963707]).[supplied by OMIM, Jun 2009]

Uniprot Description

NUSAP1: Microtubule-associated protein with the capacity to bundle and stabilize microtubules. May associate with chromosomes and promote the organization of mitotic spindle microtubules around them. Belongs to the NUSAP family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Nucleolus

Chromosomal Location of Human Ortholog: 15q15.1

Cellular Component: chromosome; cytoplasm; nucleolus; nucleus; spindle microtubule

Molecular Function: DNA binding; microtubule binding; protein binding

Biological Process: cytokinesis after mitosis; establishment of mitotic spindle localization; mitotic chromosome condensation; mitotic sister chromatid segregation; positive regulation of mitosis