Rabbit anti-Human NUDT10 Polyclonal Antibody | anti-NUDT10 antibody

NUDT10, NT (NUDT10, APS2, DIPP3A, Diphosphoinositol polyphosphate phosphohydrolase 3-alpha, Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 3-alpha, Diadenosine hexaphosphate hydrolase (AMP-forming), Nucleoside diphosphate-linked moiety X motif 10, hAps

Cat. Num. AAA6327106

• Gene Names: NUDT10; APS2; DIPP3a; DIPP3-alpha
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NUDT10; Polyclonal Antibody; NT (NUDT10; APS2; DIPP3A; Diphosphoinositol polyphosphate phosphohydrolase 3-alpha; Diadenosine 5'; 5'''-P1; P6-hexaphosphate hydrolase 3-alpha; Diadenosine hexaphosphate hydrolase (AMP-forming); Nucleoside diphosphate-linked moiety X motif 10; hAps; hAps2; anti-NUDT10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-NUDT10 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NUDT10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human NUDT10.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NUDT10 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NUDT10 Antibody detected the NUDT10 protein (arrow).)

Related Product Information for anti-NUDT10 antibody

NUDT10 belongs to a subgroup of phosphohydrolases that preferentially attack diphosphoinositol polyphosphates (Hidaka et al., 2002 [PubMed 12105228]).

Product Categories/Family for anti-NUDT10 antibody

Antibodies; NUDT19

References

Kim, S.T., et al. Prostate 70(16):1729-1738(2010). Gudmundsson, J., et al. Nat. Genet. 40(3):281-283(2008). Eeles, R.A., et al. Nat. Genet. 40(3):316-321(2008). Lamesch, P., et al. Genomics 89(3):307-315(2007). Ross, M.T., et al. Nature 434(7031):325-337(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 41393549
• NCBI GeneID: 170685
• NCBI Accession #: NP_694853.1
• NCBI GenBank Nucleotide #: NM_153183.3
• UniProt Accession #: Q8NFP7; Q86VK1; Q86VR0; A8K7D7; D3DX69
• Molecular Weight: 18,500 Da
• NCBI Official Full Name: diphosphoinositol polyphosphate phosphohydrolase 3-alpha
• NCBI Official Synonym Full Names: nudix (nucleoside diphosphate linked moiety X)-type motif 10
• NCBI Official Symbol: NUDT10
• NCBI Official Synonym Symbols: APS2; DIPP3a; DIPP3-alpha
• NCBI Protein Information: diphosphoinositol polyphosphate phosphohydrolase 3-alpha; diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 3-alpha; diadenosine hexaphosphate hydrolase (AMP-forming); nucleoside diphosphate-linked moiety X motif 10; nudix motif 10
• UniProt Protein Name: Diphosphoinositol polyphosphate phosphohydrolase 3-alpha
• Protein Family: Nudix hydrolase
• UniProt Gene Name: NUDT10
• UniProt Synonym Gene Names: APS2; DIPP3A; DIPP-3-alpha; DIPP3-alpha; hDIPP3alpha; Nudix motif 10
• UniProt Entry Name: NUD10_HUMAN

NCBI Description

This gene is a member of the nudix (nucleoside diphosphate linked moiety X)-type motif containing family. The encoded protein is a phosphohydrolase and may regulate the turnover of diphosphoinositol polyphosphates. The turnover of these high-energy diphosphoinositol polyphosphates represents a molecular switching activity with important regulatory consequences. Molecular switching by diphosphoinositol polyphosphates may contribute to the regulation of intracellular trafficking. In some populations putative prostate cancer susceptibility alleles have been identified for this gene. Alternatively spliced transcript variants, which differ only in the 5' UTR, have been found for this gene. [provided by RefSeq, Feb 2015]

Uniprot Description

NUDT10: Cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate), suggesting that it may play a role in signal transduction. Also able to catalyze the hydrolysis of dinucleoside oligophosphates, with Ap6A and Ap5A being the preferred substrates. The major reaction products are ADP and p4a from Ap6A and ADP and ATP from Ap5A. Also able to hydrolyze 5-phosphoribose 1-diphosphate. Belongs to the Nudix hydrolase family. DIPP subfamily.

Protein type: EC 3.6.1.52; EC 3.6.1.60; Hydrolase

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: cytosol

Molecular Function: metal ion binding; diphosphoinositol-polyphosphate diphosphatase activity

Biological Process: inositol phosphate metabolic process