Mouse anti-Human SMARCA4 / BRG1 Monoclonal Antibody | anti-SMARCA4 antibody

Mouse Monoclonal [clone BRG-01] (IgG2a) to Human SMARCA4 / BRG1

Cat. Num. AAA246309

• Gene Names: SMARCA4; BRG1; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein A Purified
• Synonyms: SMARCA4 / BRG1; Monoclonal Antibody; Mouse Monoclonal [clone BRG-01] (IgG2a) to Human SMARCA4 / BRG1; Anti-SMARCA4 / BRG1 Antibody (C-Terminus; clone BRG-01) IHC-plus; SMARCA4; ATP-dependent helicase SMARCA4; BAF190; BRG1-associated factor 190A; Brahma protein-like 1; BRG1; BRM/SWI2-related gene 1; HSNF2b; MRD16; Protein BRG-1; SNF2-like 4; SNF2LB; SNF2-BETA; SNF2B; SWI2; Nuclear protein GRB1; SNF2; SNF2L4; Human SMARCA4; anti-SMARCA4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: BRG-01
• Specificity: The antibody BRG-01 recognizes an epitope within C-terminal part of human Brg1, a 205 kD catalytic subunit of SWI2/SNF2-family chromatin-remodeling complexes.
• Purity/Purification: Protein A Purified
• Form/Format: PBS, 15 mM sodium azide, pH 7.4. Sourced in Ascites.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1647

• Applicable Applications for anti-SMARCA4 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (10 ug/ml); Usage: Western Blotting. Recommended dilution: 10 ug/ml. Positive control: HEK293 cells
• Target Species: Human
• Immunogen Description: Bacterially produced GST-fused protein representing 242 C-terminal amino acids of human Brg1.
• Immunogen: SMARCA4 / BRG1 antibody was raised against bacterially produced GST-fused protein representing 242 C-terminal amino acids of human Brg1.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-SMARCA4 / BRG1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-SMARCA4 / BRG1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-SMARCA4 / BRG1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 21071056
• NCBI GeneID: 6597
• NCBI Accession #: NP_003063.2
• NCBI GenBank Nucleotide #: NM_003072.3
• UniProt Accession #: P51532; O95052; Q9HBD3; B1A8Z4; B1A8Z5; B1A8Z6; B1A8Z7; E9PBR8
• Molecular Weight: 181,261 Da
• NCBI Official Full Name: transcription activator BRG1 isoform B
• NCBI Official Synonym Full Names: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• NCBI Official Symbol: SMARCA4
• NCBI Official Synonym Symbols: BRG1; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A
• NCBI Protein Information: transcription activator BRG1; SNF2-beta; SNF2-like 4; protein BRG-1; nuclear protein GRB1; brahma protein-like 1; BRM/SWI2-related gene 1; homeotic gene regulator; protein brahma homolog 1; BRG1-associated factor 190A; sucrose nonfermenting-like 4; ATP-dependent helicase SMARCA4; mitotic growth and transcription activator; global transcription activator homologous sequence
• UniProt Protein Name: Transcription activator BRG1
• Protein Family: Transcription activator
• UniProt Gene Name: SMARCA4
• UniProt Synonym Gene Names: BAF190A; BRG1; SNF2B; SNF2L4; BAF190A
• UniProt Entry Name: SMCA4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

SMARCA4: a member of the SWI/SNF nucleosome remodeling complex with ATP-dependent DNA helicase activity. SWI/SNF complexes are required for mammalian development and are mutated in ~20% of all human primary tumors. Mutated in 35% (13/37) of NSCLC and 5% (1/19) of SCLC human lung cancer cell lines . A component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. Belongs to the neural progenitor-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). Component of the BAF53 complex which acetylates histone H4 and H2A within nucleosomes. Transcriptional coactivator of nuclear hormone receptors. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription, and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1. Can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. SMARCA4, in consort with ZEB1, represses the transcription of E-cadherin and promotes the epithelial-to-mesenchymal transisition (EMT). SMARCA4 is required for stem cell maintenance in the mouse intestinal epithelium. Its loss inhibits aberrant Wnt-signalling and prevents tumorigenesis in the mouse small intestine. SMARCA4 mutations are the cause of a familial cancer syndrome predisposing to renal or extrarenal malignant rhabdoid tumors and to a variety of tumors of the central nervous system, including choroid plexus carcinoma, medulloblastoma, and central primitive neuroectodermal tumors. Rhabdoid tumors are the most aggressive and lethal malignancies occurring in early childhood. Belongs to the SNF2/RAD54 helicase family. The human protein includes 5 isoforms produced by alternative splicing.

Protein type: EC 3.6.1.-; Nuclear receptor co-regulator; Tumor suppressor; Cell cycle regulation; EC 3.6.4.-; Transcription, coactivator/corepressor; Helicase

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; heterochromatin; SWI/SNF complex; extracellular space; protein complex; membrane; perichromatin fibrils; nuclear chromatin; nucleolus; nucleus

Molecular Function: DNA-dependent ATPase activity; protein binding; nucleosomal DNA binding; androgen receptor binding; p53 binding; Tat protein binding; transcription coactivator activity; protein N-terminus binding; transcription corepressor activity; helicase activity; ATP binding

Biological Process: extracellular matrix organization and biogenesis; establishment and/or maintenance of chromatin architecture; glial cell fate determination; negative regulation of transcription from RNA polymerase II promoter, mitotic; positive regulation of transcription, DNA-dependent; cell morphogenesis; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; embryonic organ morphogenesis; epidermis morphogenesis; blastocyst hatching; forebrain development; blastocyst growth; vasculogenesis; positive regulation of Wnt receptor signaling pathway; positive regulation of DNA binding; hindbrain development; transcription, DNA-dependent; stem cell maintenance; liver development; keratinocyte differentiation; regulation of transcription from RNA polymerase II promoter; chromatin remodeling; cytosine methylation within a CG sequence; methylation-dependent chromatin silencing; nucleosome disassembly; ATP-dependent chromatin remodeling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of cell growth; negative regulation of transcription, DNA-dependent

Disease: Mental Retardation, Autosomal Dominant 16; Rhabdoid Tumor Predisposition Syndrome 2