Rabbit NUDT1 Polyclonal Antibody | anti-NUDT1 antibody

NUDT1 (Nucleoside Diphosphate-linked Moiety X Motif 1, Nudix Motif 1, MTH1) (Biotin)

Cat. Num. AAA6429930

• Gene Names: NUDT1; MTH1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: NUDT1; Polyclonal Antibody; NUDT1 (Nucleoside Diphosphate-linked Moiety X Motif 1; Nudix Motif 1; MTH1) (Biotin); anti-NUDT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes NUDT1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-NUDT1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human NUDT1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using NUDT1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded human lung using NUDT1 antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded rat lung using NUDT1 antibody at dilution of 1:200 (400x lens).)

Product Categories/Family for anti-NUDT1 antibody

Antibodies; NUDT19

NCBI and Uniprot Product Information

• NCBI GI #: 40288274
• NCBI GeneID: 4521
• NCBI Accession #: NP_002443.3
• NCBI GenBank Nucleotide #: NM_002452.3
• UniProt Accession #: P36639; Q6LES7; Q6P0Y6; Q7Z7N6; Q8IV95; Q9UBM0; Q9UBM9; A4D205
• Molecular Weight: 17,952 Da
• NCBI Official Full Name: 7,8-dihydro-8-oxoguanine triphosphatase isoform p18
• NCBI Official Synonym Full Names: nudix (nucleoside diphosphate linked moiety X)-type motif 1
• NCBI Official Symbol: NUDT1
• NCBI Official Synonym Symbols: MTH1
• NCBI Protein Information: 7,8-dihydro-8-oxoguanine triphosphatase; 2-hydroxy-dATP diphosphatase; 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase; 8-oxo-7,8-dihydroguanosine triphosphatase; 8-oxo-dGTPase; mutT human homolog 1; nucleoside diphosphate-linked moiety X motif 1; nucleosi
• UniProt Protein Name: 7,8-dihydro-8-oxoguanine triphosphatase
• Protein Family: Nudix hydrolase
• UniProt Gene Name: NUDT1
• UniProt Synonym Gene Names: MTH1; Nudix motif 1
• UniProt Entry Name: 8ODP_HUMAN

NCBI Description

Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described. [provided by RefSeq, Jul 2008]

Uniprot Description

NUDT1: Antimutagenic. Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress. Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. Belongs to the Nudix hydrolase family. 4 isoforms of the human protein are produced by alternative initiation.

Protein type: Hydrolase; EC 3.6.1.56; EC 3.6.1.55

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; nucleus; cytosol

Molecular Function: GTPase activity; snoRNA binding; m7G(5')pppN diphosphatase activity; ATP diphosphatase activity; metal ion binding; 8-oxo-7,8-dihydroguanine triphosphatase activity

Biological Process: DNA protection; dGTP catabolic process; dATP catabolic process; nucleobase, nucleoside and nucleotide metabolic process; purine nucleotide catabolic process; response to oxidative stress; DNA repair