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Immunofluorescence (IF) (Immunofluorescence staining of GNL3 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human GNL3 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to nucleus..)

Rabbit anti-Human Nucleostemin Polyclonal Antibody | anti-GNL3 antibody

Anti-Nucleostemin Antibody, Rabbit Polyclonal

Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
Nucleostemin; Polyclonal Antibody; Anti-Nucleostemin Antibody; Rabbit Polyclonal; GNL3/Nucleostemin Antibody; Rabbit PAb; Antigen Affinity Purified; guanine nucleotide binding protein-like 3 (nucleolar); Anti-C77032 Antibody; Anti-E2IG3 Antibody; Anti-NNP47 Antibody; Anti-NS Antibody; anti-GNL3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human GNL3/Nucleostemin
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-GNL3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
ICC/IF: 1:1000-1:5000
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human GNL3/Nucleostemin
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human GNL3/Nucleostemin, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of GNL3 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human GNL3 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to nucleus..)

Immunofluorescence (IF) (Immunofluorescence staining of GNL3 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human GNL3 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to nucleus..)

Western Blot (WB)

(Anti-GNL3 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:62 kDaObserved band size:62 kDa(We are unsure as to the identity of these extra bands.))

Western Blot (WB) (Anti-GNL3 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:62 kDaObserved band size:62 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-GNL3 antibody
GNL3 (G Protein Nucleolar 3, also known as NS) is a Protein Coding gene. GNL3 belongs to the TRAFAC class YlqF/YawG GTPase family. The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. GNL3 is widely expressed in the urinary bladder, prostate, and other tissues. GNL3 protein has been recently documented to be a nucleolar protein that was abundantly expressed in stem cells and cancer cells. NS may contribute to sorafenib resistance in HCC cells through the modulation of the p53 pathway and Bcl-2 proteins. That the combination of silencing NS expression and sorafenib treatment is a promising therapeutic strategy in the treatment of HCC. GNL3 is a potential genetic modifier for knee osteoarthritis (KOA) development.

NCBI and Uniprot Product Information

UniProt Accession #
UniProt Protein Name
Guanine nucleotide-binding protein-like 3
UniProt Gene Name
GNL3
UniProt Synonym Gene Names
E2IG3; NS
UniProt Entry Name
GNL3_HUMAN

Uniprot Description

GNL3: May be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis. May interact with p53/TP53 via its basic domain. Increased levels in lung tissue in cancer patients. Belongs to the MMR1/HSR1 GTP-binding protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Cell cycle regulation; Nucleolus; RNA-binding

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: nucleoplasm; extracellular space; membrane; nucleolus; nucleus

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: cell proliferation; metabolic process; ribosome biogenesis and assembly; regulation of cell proliferation

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Product Notes

The GNL3 gnl3 (Catalog #AAA8100608) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Nucleostemin Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleostemin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500-1:1000 ICC/IF: 1:1000-1:5000. Researchers should empirically determine the suitability of the GNL3 gnl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nucleostemin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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