Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Nucleoporin 133kDa (NUP133) Polyclonal Antibody | anti-NUP133 antibody
Polyclonal Antibody to Nucleoporin 133kDa (NUP133)
Gene Names
Nup133; mermaid
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Nucleoporin 133kDa (NUP133); Polyclonal Antibody; Polyclonal Antibody to Nucleoporin 133kDa (NUP133); anti-NUP133 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against NUP133. It has been selected for its ability to recognize NUP133 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LSLGS AVNSPVLFSP AGRRSSVSSR GTPTRIFPHH SISESVNYDV RVFGSSLPVK IMEALTMAEA DEQLSVHVDE GGWACLVCTE KLLIWKIAVS PVTKLSVCKE LQLPPSDFHG SADLVALSYA ATSGEVHSVQ AVSVMVATKE GSIRYWPSLA REDTYSDTCV DLGGEKMCRF LTAVQGGSFI LSSVGSQLVR
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LSLGS AVNSPVLFSP AGRRSSVSSR GTPTRIFPHH SISESVNYDV RVFGSSLPVK IMEALTMAEA DEQLSVHVDE GGWACLVCTE KLLIWKIAVS PVTKLSVCKE LQLPPSDFHG SADLVALSYA ATSGEVHSVQ AVSVMVATKE GSIRYWPSLA REDTYSDTCV DLGGEKMCRF LTAVQGGSFI LSSVGSQLVR
Sequence Length
1156
Applicable Applications for anti-NUP133 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant NUP133 (Leu36~His240) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2051927
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Heart tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Heart tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
128,620 Da
NCBI Official Full Name
nuclear pore complex protein Nup133
NCBI Official Synonym Full Names
nucleoporin 133
NCBI Official Symbol
Nup133
NCBI Official Synonym Symbols
mermaid
NCBI Protein Information
nuclear pore complex protein Nup133
UniProt Protein Name
Nuclear pore complex protein Nup133
Protein Family
UniProt Gene Name
Nup133
Uniprot Description
NUP133: Involved in poly(A)+ RNA transport. Belongs to the nucleoporin Nup133 family.
Protein type: Nucleoporin
Chromosomal Location of Human Ortholog: 8|8 E2
Cellular Component: chromosome; chromosome, centromeric region; condensed chromosome kinetochore; kinetochore; membrane; nuclear membrane; nuclear pore; nucleus; outer kinetochore of condensed chromosome
Molecular Function: nucleocytoplasmic transporter activity; structural constituent of nuclear pore
Biological Process: chromatin organization; mRNA export from nucleus; mRNA transport; neural tube development; neurogenesis; nuclear pore distribution; nuclear pore organization and biogenesis; paraxial mesoderm development; protein import into nucleus; protein transport; regulation of transcription, DNA-templated; RNA export from nucleus; transport
Similar Products
Product Notes
The NUP133 nup133 (Catalog #AAA2017195) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Nucleoporin 133kDa (NUP133) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleoporin 133kDa (NUP133) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NUP133 nup133 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LSLGS AVNSPVLFSP AGRRSSVSSR GTPTRIFPHH SISESVNYDV RVFGSSLPVK IMEALTMAEA DEQLSVHVDE GGWACLVCTE KLLIWKIAVS PVTKLSVCKE LQLPPSDFHG SADLVALSYA ATSGEVHSVQ AVSVMVATKE GSIRYWPSLA REDTYSDTCV DLGGEKMCRF LTAVQGGSFI LSSVGSQLVR. It is sometimes possible for the material contained within the vial of "Nucleoporin 133kDa (NUP133), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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