Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Nucleoporin 88kDa (NUP88) Polyclonal Antibody | anti-NUP88 antibody
Polyclonal Antibody to Nucleoporin 88kDa (NUP88)
Gene Names
Nup88; Nup84; Prei2
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Nucleoporin 88kDa (NUP88); Polyclonal Antibody; Polyclonal Antibody to Nucleoporin 88kDa (NUP88); anti-NUP88 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against NUP88. It has been selected for its ability to recognize NUP88 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TI PIAERFFTSS TSLTLKHAAW YPSEMLDPHI VLLTSDNVIR IYSLREPQTP TKVIVLSEAE EESLILNKGR AYTASLGETA VAFDFGPLVT VSKNMFEQKD REAVAYPLYI LYENGETFLT YVSLLHSPGN IGKLLGPLPM HPAAEDNYGY DACAILCLPC VPNILVIATE SGMLYHCVVL EGEEDDDQTL EKSWDPRADL IPSLYVFECV ELELALKLAS AEDDPFASDF SCPIKLHRDP KCPSRYHCSH EAGVHSVGLT WI
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TI PIAERFFTSS TSLTLKHAAW YPSEMLDPHI VLLTSDNVIR IYSLREPQTP TKVIVLSEAE EESLILNKGR AYTASLGETA VAFDFGPLVT VSKNMFEQKD REAVAYPLYI LYENGETFLT YVSLLHSPGN IGKLLGPLPM HPAAEDNYGY DACAILCLPC VPNILVIATE SGMLYHCVVL EGEEDDDQTL EKSWDPRADL IPSLYVFECV ELELALKLAS AEDDPFASDF SCPIKLHRDP KCPSRYHCSH EAGVHSVGLT WI
Sequence Length
741
Applicable Applications for anti-NUP88 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant NUP88 (Thr159~Ile422) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2052439
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Lung tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Lung tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
83,585 Da
NCBI Official Full Name
nuclear pore complex protein Nup88
NCBI Official Synonym Full Names
nucleoporin 88
NCBI Official Symbol
Nup88
NCBI Official Synonym Symbols
Nup84; Prei2
NCBI Protein Information
nuclear pore complex protein Nup88
UniProt Protein Name
Nuclear pore complex protein Nup88
UniProt Gene Name
Nup88
UniProt Synonym Gene Names
Nup84
NCBI Description
localized to the cytoplasmic face of the nuclear pore complex; associates with nuclear pore filament O-linked glycoprotein CAN/Nup214 [RGD, Feb 2006]
Uniprot Description
NUP88: Essential component of nuclear pore complex
Protein type: Motility/polarity/chemotaxis; Nucleoporin
Chromosomal Location of Human Ortholog: 10q24
Cellular Component: nuclear pore; nucleoplasm
Molecular Function: protein binding; transporter activity
Biological Process: mitotic cell cycle; mRNA export from nucleus; protein import into nucleus; ribosomal large subunit export from nucleus; ribosomal small subunit export from nucleus
Similar Products
Product Notes
The NUP88 nup88 (Catalog #AAA2016887) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Nucleoporin 88kDa (NUP88) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleoporin 88kDa (NUP88) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NUP88 nup88 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TI PIAERFFTSS TSLTLKHAAW YPSEMLDPHI VLLTSDNVIR IYSLREPQTP TKVIVLSEAE EESLILNKGR AYTASLGETA VAFDFGPLVT VSKNMFEQKD REAVAYPLYI LYENGETFLT YVSLLHSPGN IGKLLGPLPM HPAAEDNYGY DACAILCLPC VPNILVIATE SGMLYHCVVL EGEEDDDQTL EKSWDPRADL IPSLYVFECV ELELALKLAS AEDDPFASDF SCPIKLHRDP KCPSRYHCSH EAGVHSVGLT WI. It is sometimes possible for the material contained within the vial of "Nucleoporin 88kDa (NUP88), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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