Western Blot (WB)
(Western Blot: Sample: Human Hela cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Human NUP133 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Human Nucleoporin 133kDa Polyclonal Antibody | anti-NUP133 antibody
Nucleoporin 133kDa (NUP133) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Human Hela cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Human NUP133 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human Hela cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Human NUP133 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
NCBI and Uniprot Product Information
NCBI Description
The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells. [provided by RefSeq, Jul 2008]
Uniprot Description
NUP133: Involved in poly(A)+ RNA transport. Belongs to the nucleoporin Nup133 family.
Protein type: Nucleoporin
Chromosomal Location of Human Ortholog: 1q42.13
Cellular Component: condensed chromosome kinetochore; cytosol; membrane; nuclear envelope; nuclear membrane; nuclear pore; outer kinetochore of condensed chromosome
Molecular Function: nucleocytoplasmic transporter activity; protein binding; structural constituent of nuclear pore
Biological Process: chromatin organization; mitotic nuclear envelope disassembly; mRNA export from nucleus; neural tube development; neurogenesis; nuclear pore distribution; nuclear pore organization and biogenesis; paraxial mesoderm development; protein import into nucleus; protein sumoylation; regulation of cellular response to heat; regulation of glycolysis; regulation of transcription, DNA-templated; sister chromatid cohesion; tRNA export from nucleus; viral process; viral transcription
Research Articles on NUP133
Similar Products
Product Notes
The NUP133 nup133 (Catalog #AAA2032358) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nucleoporin 133kDa (NUP133) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleoporin 133kDa can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NUP133 nup133 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nucleoporin 133kDa, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
