Western Blot (WB)
(Western blot analysis NUP133 using LOVO whole cell lysates)
Rabbit NUP133 Polyclonal Antibody | anti-NUP133 antibody
NUP133 Antibody
Gene Names
NUP133; hNUP133
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NUP133; Polyclonal Antibody; NUP133 Antibody; 133 kDa nucleoporin; FLJ10814; hNUP 133; hNUP133; MGC21133; NU133_HUMAN; Nuclear pore complex protein Nup133; Nucleoporin 133kDa; Nucleoporin NUP133; NUP 133; OTTHUMP00000061095; anti-NUP133 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
NUP133 antibody detects endogenous levels of total NUP133
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1156
Applicable Applications for anti-NUP133 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human NUP133
Subcellular Location
Nucleus > Nuclear Pore Complex. Chromosome > Centromere > Kinetochore. Located on both the cytoplasmic and nuclear sides of the nuclear pore. During mitosis, localizes to the kinetochores.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (90%), Zebrafish (89%), Bovine (90%), Horse (90%), Sheep (90%), Rabbit (90%), Dog (100%), Chicken (90%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis NUP133 using LOVO whole cell lysates)
Western Blot (WB)
(Western blot analysis NUP133 using LOVO whole cell lysates)
Related Product Information for anti-NUP133 antibody
Function: Involved in poly(A)+ RNA transport.
Subunit Structure: Forms part of the Nup160 subcomplex in the nuclear pore which is composed of NUP160, NUP133, NUP107 and Nup96. This complex plays a role in RNA export and in tethering Nup98 and NUP153 to the nucleus.
Similarity: Belongs to the nucleoporin Nup133 family.
Subunit Structure: Forms part of the Nup160 subcomplex in the nuclear pore which is composed of NUP160, NUP133, NUP107 and Nup96. This complex plays a role in RNA export and in tethering Nup98 and NUP153 to the nucleus.
Similarity: Belongs to the nucleoporin Nup133 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 129 kDa
Predicted: 129 kDa
Predicted: 129 kDa
NCBI Official Full Name
nuclear pore complex protein Nup133
NCBI Official Synonym Full Names
nucleoporin 133
NCBI Official Symbol
NUP133
NCBI Official Synonym Symbols
hNUP133
NCBI Protein Information
nuclear pore complex protein Nup133
UniProt Protein Name
Nuclear pore complex protein Nup133
Protein Family
UniProt Gene Name
NUP133
NCBI Description
The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells. [provided by RefSeq, Jul 2008]
Uniprot Description
Involved in poly(A)+ RNA transport.
Research Articles on NUP133
Similar Products
Product Notes
The NUP133 nup133 (Catalog #AAA9610023) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NUP133 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NUP133 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the NUP133 nup133 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUP133, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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