Western Blot (WB)
(Western Blot: Lane1: Mouse Liver Tissue; Lane2: Human Jurkat Cells; Lane3: Human K562 Cells.)
Rabbit anti-Mouse Nucleophosmin (NPM) Polyclonal Antibody | anti-NPM antibody
Polyclonal Antibody to Nucleophosmin (NPM)
MGHHHHHHSGSEF- DLKPSTPRS KGQESFKKQE KTPKTPKGPS SVEDIKAKMQ ASIEKGGSLP KVEAKFINYV KNCFRMTDQE AIQDLWQWRK SL
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Lane1: Mouse Liver Tissue; Lane2: Human Jurkat Cells; Lane3: Human K562 Cells.)
Western Blot (WB)
(Western Blot: Lane1: Mouse Liver Tissue; Lane2: Human Jurkat Cells; Lane3: Human K562 Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant NPM, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant NPM, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Pancreas Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Pancreas Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Adipose Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Adipose Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Intestine Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Intestine Tissue)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: NPM knockout Jurkat cell lysate; Predicted MW: 33kd Observed MW: 38kd Primary Ab: 1ug/ml Rabbit Anti-Mouse NPM Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: NPM knockout Jurkat cell lysate; Predicted MW: 33kd Observed MW: 38kd Primary Ab: 1ug/ml Rabbit Anti-Mouse NPM Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
Uniprot Description
NPM1: a nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described.
Protein type: Nucleolus; Oncoprotein; RNA-binding; Translation
Chromosomal Location of Human Ortholog: 11|11 A4
Cellular Component: centrosome; cytoplasm; cytoskeleton; cytosol; focal adhesion; granular component; intracellular ribonucleoprotein complex; large ribosomal subunit; membrane; nuclear matrix; nuclear speck; nucleolus; nucleoplasm; nucleus; protein complex; small ribosomal subunit; spindle pole centrosome
Molecular Function: activating transcription factor binding; ATP binding; DNA binding; enzyme binding; histone binding; NF-kappaB binding; nucleic acid binding; p53 binding; phosphatidylinositol-3,4,5-trisphosphate binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase B binding; protein kinase binding; protein kinase inhibitor activity; protein N-terminus binding; ribosomal large subunit binding; ribosomal small subunit binding; RNA binding; rRNA binding; Tat protein binding; transcription coactivator activity; transcription factor binding; unfolded protein binding
Biological Process: cell aging; cell growth; cell volume homeostasis; cellular response to UV; centrosome cycle; cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); DNA repair; negative regulation of apoptosis; negative regulation of cardiac muscle cell apoptotic process; negative regulation of cell proliferation; negative regulation of centrosome duplication; negative regulation of epithelial cell proliferation; negative regulation of gene expression; negative regulation of neuron apoptosis; negative regulation of nuclear mRNA splicing, via spliceosome; negative regulation of protein kinase activity by regulation of protein phosphorylation; nucleocytoplasmic transport; nucleosome assembly; positive regulation of catalytic activity; positive regulation of cell cycle G2/M phase transition; positive regulation of cell proliferation; positive regulation of cellular biosynthetic process; positive regulation of centrosome duplication; positive regulation of DNA metabolic process; positive regulation of DNA replication; positive regulation of DNA-directed DNA polymerase activity; positive regulation of NF-kappaB transcription factor activity; positive regulation of protein kinase activity; positive regulation of protein localization to nucleolus; positive regulation of protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; positive regulation of translation; posttranscriptional regulation of gene expression; protein destabilization; protein homooligomerization; protein localization; protein oligomerization; protein stabilization; regulation of cell cycle; regulation of centriole replication; regulation of centrosome duplication; regulation of DNA damage response, signal transduction by p53 class mediator; regulation of endodeoxyribonuclease activity; regulation of neuron apoptosis; regulation of protein stability; response to stress; ribosomal large subunit biogenesis; ribosomal large subunit export from nucleus; ribosomal small subunit biogenesis and assembly; ribosomal small subunit export from nucleus; rRNA export from nucleus; rRNA transcription
Research Articles on NPM
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Product Notes
The NPM npm1 (Catalog #AAA2002212) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Nucleophosmin (NPM) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleophosmin (NPM) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NPM npm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- DLKPSTPRS KGQESFKKQE KTPKTPKGPS SVEDIKAKMQ ASIEKGGSLP KVEAKFINYV KNCFRMTDQE AIQDLWQWRK SL. It is sometimes possible for the material contained within the vial of "Nucleophosmin (NPM), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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