Rabbit Nucleophosmin Polyclonal Antibody | anti-NPM1 antibody

Anti-Nucleophosmin antibody

Cat. Num. AAA176054

• Gene Names: NPM1; B23; NPM
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen affinity purified.
• Synonyms: Nucleophosmin; Polyclonal Antibody; Anti-Nucleophosmin antibody; nucleophosmin(nucleolar phosphoprotein B23; numatrin); B23 antibody; MGC104254 antibody; NO38 antibody; NPM antibody; NPM_HUMAN antibody; NPM1 antibody; Nucleolar Phosphoprotein B23 antibody; Nucleolar protein NO38 antibody; Nucleophosmin(nucleolar phosphoprotein B23 numatrin) antibody; Nucleophosmin antibody; Nucleophosmin/nucleoplasmin family member 1 antibody; Numatrin antibody; OTTHUMP00000161024 antibody; OTTHUMP00000161025 antibody; OTTHUMP00000223397 antibody; OTTHUMP00000223398 antibody; anti-NPM1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 294

• Applicable Applications for anti-NPM1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Nucleophosmin (22-41aa ELKADKDYHFKVDNDENEHQ), identical to the related rat and mouse sequences.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-Nucleophosmin antibody, MBS176054, Western blottingLane 1: Rat Testis Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: HELA Cell LysateLane 4: U87 Cell LysateLane 5: A549 Cell LysateLane6: SMMC Cell Lysate )

Immunohistochemistry (IHC)

(Anti-Nucleophosmin antibody, MBS176054, IHC(P)IHC(P): Human Intestinal Cancer Tissue )

Immunocytochemistry (ICC)

(Anti-Nucleophosmin antibody, MBS176054, ICCICC: HELA Cell )

Immunohistochemistry (IHC)

(Anti-Nucleophosmin antibody, MBS176054, IHC(P)IHC(P): Rat Spleen Tissue )

Related Product Information for anti-NPM1 antibody

Description: Rabbit IgG polyclonal antibody for Nucleophosmin(NPM1) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: NPM1(Nucleophosmin/Nucleoplasmin family, member1), also known as NPM, nucleolar phosphoprotein B23 or numatrin, is a protein that in humans is encoded by the NPM1 gene. The NPM1 gene maps to chromosome 5q35. Chan et al.(1989) found that nucleophosmin is a nucleolar phosphoprotein that is more abundant in tumor cells than in normal resting cells. Stimulation of the growth of normal cells, e.g., mitogen activation of B lymphocytes, was accompanied by an increase in nucleophosmin protein level. They stated that nucleophosmin is likely involved in the assembly of ribosomal proteins into ribosomes. Electron microscopic study indicated that nucleophosmin is concentrated in the granular region of the nucleolus, where ribosome assembly occurs.

NCBI and Uniprot Product Information

• NCBI GI #: 114762
• NCBI GeneID: 4869
• UniProt Accession #: P06748; P08693; Q12826; Q13440; Q13441; Q14115; Q5EU94; Q5EU95; Q5EU96; A8K3N7; B5BU00; D3DQL6
• Molecular Weight: 28,400 Da
• NCBI Official Full Name: Nucleophosmin
• NCBI Official Synonym Full Names: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• NCBI Official Symbol: NPM1
• NCBI Official Synonym Symbols: B23; NPM
• NCBI Protein Information: nucleophosmin; nucleolar protein NO38; nucleophosmin/nucleoplasmin family, member 1
• UniProt Protein Name: Nucleophosmin
• Protein Family: Nucleophosmin
• UniProt Gene Name: NPM1
• UniProt Synonym Gene Names: NPM; NPM
• UniProt Entry Name: NPM_HUMAN

NCBI Description

This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]

Uniprot Description

NPM1: a nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described.

Protein type: RNA-binding; Translation; Oncoprotein; Nucleolus

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: nucleoplasm; centrosome; focal adhesion; membrane; cytoplasm; nucleolus; spindle pole centrosome; ribonucleoprotein complex; cytosol; nucleus

Molecular Function: NF-kappaB binding; protein binding; protein homodimerization activity; protein kinase inhibitor activity; histone binding; protein heterodimerization activity; RNA binding; Tat protein binding; transcription coactivator activity; unfolded protein binding; protein kinase binding; ribosomal small subunit binding; ribosomal large subunit binding

Biological Process: regulation of centriole replication; positive regulation of translation; viral reproduction; centrosome cycle; cell aging; nucleocytoplasmic transport; signal transduction; DNA repair; protein oligomerization; activation of NF-kappaB transcription factor; intracellular protein transport; nucleosome assembly; negative regulation of cell proliferation; protein localization; regulation of endodeoxyribonuclease activity; DNA replication-independent nucleosome assembly at centromere; ribosome assembly; response to stress; negative regulation of apoptosis