Mouse anti-Human Nucleophosmin 1 (NPM1) Monoclonal Antibody | anti-NPM1 antibody

Anti-Nucleophosmin 1 (NPM1) Antibody

Cat. Num. AAA418405

• Gene Names: NPM1; B23; NPM
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Protein A affinity chromatography.
• Synonyms: Nucleophosmin 1 (NPM1); Monoclonal Antibody; Anti-Nucleophosmin 1 (NPM1) Antibody; anti-NPM1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Purity/Purification: Protein A affinity chromatography.
• Form/Format: Liquid
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 294

• Applicable Applications for anti-NPM1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Ni-NTA purified recombinant human NPM1 expressed in E Coli strain BL21 (DE3).
• Storage Buffer: PBS, pH 7.4 with 0.05% sodium azide.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Immunohistochemistry (IHC)

Related Product Information for anti-NPM1 antibody

Nucleophosmin (NPM), also named B23 or NO38, is a major nucleolar protein which is 20 times more abundant in tumor or proliferating cells than in normal resting cells. NPM1 has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication and molecular chaperoning activities. The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase.

References

1.Morris SW, et al Science 1994 Mar 4; 263 (5151):1281-4 2.Yoneda-Kato N Oncogene 1996 Jan 18;12 (2):265-75

NCBI and Uniprot Product Information

• NCBI GI #: 10835063
• NCBI GeneID: 4869
• NCBI Accession #: NP_002511.1
• NCBI GenBank Nucleotide #: NM_002520.6
• UniProt Accession #: P06748; P08693; Q12826; Q13440; Q13441; Q14115; Q5EU94; Q5EU95; Q5EU96; A8K3N7; B5BU00; D3DQL6
• Molecular Weight: 28,400 Da
• NCBI Official Full Name: nucleophosmin isoform 1
• NCBI Official Synonym Full Names: nucleophosmin
• NCBI Official Symbol: NPM1
• NCBI Official Synonym Symbols: B23; NPM
• NCBI Protein Information: nucleophosmin
• UniProt Protein Name: Nucleophosmin
• Protein Family: Nucleophosmin
• UniProt Gene Name: NPM1
• UniProt Synonym Gene Names: NPM; NPM

NCBI Description

This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]

Uniprot Description

NPM1: a nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described.

Protein type: Nucleolus; Oncoprotein; RNA-binding; Translation

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: centrosome; cytoplasm; cytosol; focal adhesion; membrane; nucleolus; nucleoplasm; nucleus; ribonucleoprotein complex; spindle pole centrosome

Molecular Function: histone binding; NF-kappaB binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein kinase inhibitor activity; ribosomal large subunit binding; ribosomal small subunit binding; RNA binding; Tat protein binding; transcription coactivator activity; unfolded protein binding

Biological Process: activation of NF-kappaB transcription factor; cell aging; centrosome cycle; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA repair; DNA replication-independent nucleosome assembly at centromere; intracellular protein transport; negative regulation of apoptosis; negative regulation of cell proliferation; nucleocytoplasmic transport; nucleosome assembly; positive regulation of cell proliferation; positive regulation of transcription, DNA-dependent; positive regulation of translation; protein localization; protein oligomerization; regulation of centriole replication; regulation of endodeoxyribonuclease activity; response to stress; ribosome assembly