Rabbit anti-Human Nuclear Receptor NR4A1 Polyclonal Antibody | anti-NR4A1 antibody

Nuclear Receptor NR4A1 (Ab-351) Antibody

Cat. Num. AAA9403287

• Gene Names: NR4A1; HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Nuclear Receptor NR4A1; Polyclonal Antibody; Nuclear Receptor NR4A1 (Ab-351) Antibody; Early response protein NAK1; GFRP; GFRP1; HMR; N10; anti-NR4A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total Nuclear Receptor NR4A1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 611

• Applicable Applications for anti-NR4A1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:3000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide
• Immunogen Description: Synthesized non-phosphopeptide derived from human Nuclear Receptor NR4A1 around the phosphorylation site of serine 351 (L-P-S(p)-K-P).
• Target Name: Nuclear Receptor NR4A1
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Nuclear Receptor NR4A1 (Ab-351) antibody.)

Western Blot (WB)

(Western blot analysis of extracts from COS-7 cells, using Nuclear Receptor NR4A1 (Ab-351) antibody.)

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells (Lane 2), using Nuclear Receptor NR4A1 (Ab-351) antiobdy. The lane on the left is treated with synthesized peptide.)

Related Product Information for anti-NR4A1 antibody

Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' By similarity. May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation.Nakai A., Mol. Endocrinol. 4:1438-1443(1990).Chang C., J. Steroid Biochem. 34:391-395(1989).The MGC Project Team; Genome Res. 14:2121-2127(2004).

Product Categories/Family for anti-NR4A1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 320202956
• NCBI GeneID: 3164
• NCBI Accession #: NP_001189162.1
• NCBI GenBank Nucleotide #: NM_001202233.1
• UniProt Accession #: P22736; Q15627; Q53Y00; Q6IBU8; B4DML7
• Molecular Weight: 33,754 Da
• NCBI Official Full Name: nuclear receptor subfamily 4 group A member 1 isoform 2
• NCBI Official Synonym Full Names: nuclear receptor subfamily 4, group A, member 1
• NCBI Official Symbol: NR4A1
• NCBI Official Synonym Symbols: HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77
• NCBI Protein Information: nuclear receptor subfamily 4 group A member 1
• UniProt Protein Name: Nuclear receptor subfamily 4 group A member 1
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR4A1
• UniProt Synonym Gene Names: GFRP1; HMR; NAK1; Nur77
• UniProt Entry Name: NR4A1_HUMAN

NCBI Description

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

Nur77: an orphan nuclear receptor and immediate-early gene that regulates cellular proliferation, apoptosis, inflammation, and glucose metabolism. Induced by exercise in muscle and is a functional regulator of glucose metabolism in skeletal muscle. Its level decreases in the muscle of obese insulin-resistant men. Acts concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. A mediator of TCR-directed thymocyte apoptosis. TCR-signaling induces a FAIM/Akt/Nur77 signaling pathway that is critical for modulating apoptosis in developing thymocytes. A physiological substrate of the MEK-ERK-RSK cascade that modulates nuclear export and intracellular translocation during T cell death. Binds DNA as a monomer. Interacts with GADD45GIP1. Overexpression of Nur77 induces the expression of both p300 and HDAC1. Acetylation by p300 and HDAC1 may regulate the rapid turnover of Nur77 protein.

Protein type: Apoptosis; Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity

Biological Process: epidermal growth factor receptor signaling pathway; transcription initiation from RNA polymerase II promoter; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; intracellular receptor-mediated signaling pathway; nerve growth factor receptor signaling pathway; signal transduction; cell migration during sprouting angiogenesis; innate immune response; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; gene expression