Rabbit anti-Human CCNC Polyclonal Antibody | anti-CCNC antibody

CCNC, NT (CCNC, Cyclin-C, SRB11 homolog) (HRP)

Cat. Num. AAA6282315

• Gene Names: CCNC; CycC
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: CCNC; Polyclonal Antibody; NT (CCNC; Cyclin-C; SRB11 homolog) (HRP); SRB11 homolog; anti-CCNC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CCNC antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: CCNC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CCNC.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(CCNC Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the CCNC antibody detected the CCNC protein (arrow).)

Flow Cytometry (FC/FACS)

(CCNC Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CCNC antibody

Antibodies; Cyclin

References

Miyata, Y., et al. Stem Cells 28(2):308-317(2010). Makkonen, K.M., et al. J. Mol. Biol. 393(2):261-271(2009). Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Katona, R.L., et al. Acta. Biol. Hung. 58(1):133-137(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 61676090
• NCBI GeneID: 892
• NCBI Accession #: NP_005181.2
• NCBI GenBank Nucleotide #: NM_005190
• UniProt Accession #: P24863; Q9H543; B4DPZ1
• Molecular Weight: ~55 kDa
• NCBI Official Full Name: Homo sapiens cyclin C (CCNC), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: cyclin C
• NCBI Official Symbol: CCNC
• NCBI Official Synonym Symbols: CycC
• NCBI Protein Information: cyclin-C; hSRB11; SRB11 homolog
• UniProt Protein Name: Cyclin-C
• UniProt Gene Name: CCNC
• UniProt Synonym Gene Names: hSRB11
• UniProt Entry Name: CCNC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the cyclin family of proteins. The encoded protein interacts with cyclin-dependent kinase 8 and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this gene peaks in the G1 phase of the cell cycle. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNC: Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Belongs to the cyclin family. Cyclin C subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleoplasm; Srb-mediator complex; DNA-directed RNA polymerase II, holoenzyme

Molecular Function: protein binding; protein kinase binding

Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; regulation of cyclin-dependent protein kinase activity