Rabbit Nuclear matrix protein p84/THOC1 Polyclonal Antibody | anti-THOC1 antibody

Nuclear matrix protein p84/THOC1 Rabbit pAb

Cat. Num. AAA4755550

• Gene Names: THOC1; P84; HPR1; P84N5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Affinity purified
• Synonyms: Nuclear matrix protein p84/THOC1; Polyclonal Antibody; Nuclear matrix protein p84/THOC1 Rabbit pAb; THO complex 1; P84; HPR1; P84N5; anti-THOC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression.
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine(pH7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-THOC1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:2000; IHC: 1:20; ICC/IF: 1:20
• Immunogen: A synthetic peptide of human Nuclear Matrix Protein p84
• Conjugation: Unconjugated
• Molecular Function: Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Regulates transcriptional elongation of a subset of genes. Involved in genome stability by preventing co-transcriptional R-loop formation.Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1.
• Modification: Unmodified
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot detection of Nuclear Matrix Protein p84 in 293T,PC-12, 3T3 using Nuclear Matrix Protein p84 Rabbit pAb.Predicted band size:76kDa.Observed band size:84kDa.)

Product Categories/Family for anti-THOC1 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 154448889
• NCBI GeneID: 9984
• NCBI Accession #: NP_005122.2
• NCBI GenBank Nucleotide #: NM_005131
• UniProt Accession #: Q96FV9; Q15219; Q64I72; Q64I73; B2RBP6
• Molecular Weight: 43,359 Da
• NCBI Official Full Name: Homo sapiens THO complex 1 (THOC1), mRNA
• NCBI Official Synonym Full Names: THO complex 1
• NCBI Official Symbol: THOC1
• NCBI Official Synonym Symbols: P84; HPR1; P84N5
• NCBI Protein Information: THO complex subunit 1; hTREX84; nuclear matrix protein p84; tho1
• UniProt Protein Name: THO complex subunit 1
• UniProt Gene Name: THOC1
• UniProt Synonym Gene Names: HPR1; Tho1; p84N5
• UniProt Entry Name: THOC1_HUMAN

NCBI Description

HPR1 is part of the TREX (transcription/export) complex, which includes TEX1 (MIM 606929), THO2 (MIM 300395), ALY (MIM 604171), and UAP56 (MIM 142560).[supplied by OMIM, Nov 2010]

Uniprot Description

Tho1: a protein associated the nuclear matrix. Part of the TREX (transcription/export) complex. The TREX complex is recruited to transcribed genes and travels with the RNA polymerase during elongation. It may physically link proteins that function in transcription and in RNA export.

Protein type: Spliceosome

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: nucleoplasm; intercellular bridge; nuclear matrix; cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding; DNA binding; RNA binding

Biological Process: RNA processing; mRNA export from nucleus; transcription, DNA-dependent; negative regulation of isotype switching to IgA isotypes; apoptosis; RNA splicing; positive regulation of RNA elongation; regulation of DNA recombination; intronless viral mRNA export from host nucleus; signal transduction; mRNA processing; regulation of RNA elongation; replication fork processing