Sheep Anti-Thrombin III ELISA Kit | AT-III elisa kit

Sheep Anti-Thrombin III ELISA Kit

Cat. Num. AAA739263

• Gene Names: Serpinc1; At3; At-3; ATIII; AI114908
• Reactivity: Sheep
• Synonyms: Anti-Thrombin III; Sheep Anti-Thrombin III ELISA Kit; AT-III elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This AT-III ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep AT-III. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AT-III elisa kit

Principle of the Assay: AT-III ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AT-III antibody and an AT-III-HRP conjugate. The assay sample and buffer are incubated together with AT-III-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AT-III concentration since AT-III from samples and AT-III-HRP conjugate compete for the anti-AT-III antibody binding site. Since the number of sites is limited, as more sites are occupied by AT-III from the sample, fewer sites are left to bind AT-III-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AT-III concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 258959
• NCBI GeneID: 11905
• Molecular Weight: 52,004 Da
• NCBI Official Full Name: antithrombin III
• NCBI Official Synonym Full Names: serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1
• NCBI Official Symbol: Serpinc1
• NCBI Official Synonym Symbols: At3; At-3; ATIII; AI114908
• NCBI Protein Information: antithrombin-III; serpin C1; anti-thrombin 3; serpin peptidase inhibitor, clade C, member 1; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
• UniProt Protein Name: Antithrombin-III
• UniProt Gene Name: Serpinc1
• UniProt Synonym Gene Names: At3; ATIII
• UniProt Entry Name: ANT3_MOUSE

Uniprot Description

SERPINC1: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D). AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin- binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. Belongs to the serpin family.

Protein type: Secreted; Secreted, signal peptide; Inhibitor

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; serine-type endopeptidase inhibitor activity; protease binding; protease inhibitor activity

Biological Process: hemostasis; negative regulation of inflammatory response; negative regulation of peptidase activity; blood coagulation; response to nutrient