Rabbit NTRK2 Polyclonal Antibody | anti-NTRK2 antibody

NTRK2 Polyclonal Antibody

Cat. Num. AAA126700

• Gene Names: NTRK2; TRKB; trk-B; GP145-TrkB
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: NTRK2; Polyclonal Antibody; NTRK2 Polyclonal Antibody; GP145-TrkB; TRKB; anti-NTRK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 822

• Applicable Applications for anti-NTRK2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human NTRK2
• Calculated Molecular Weight: 90kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using NTRK2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)

Related Product Information for anti-NTRK2 antibody

The family of Trk receptor tyrosine kinases consists of TrkA, TrkB, and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3 (1). Neurotrophin signaling through these receptors regulates a number of physiological processes, such as cell survival, proliferation, neural development, and axon and dendrite growth and patterning (1). In the adult nervous system, the Trk receptors regulate synaptic strength and plasticity. TrkA regulates proliferation and is important for development and maturation of the nervous system (2). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade (3,4). Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at these sites reflects TrkA kinase activity (3-6). Point mutations, deletions, and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA (7-10). TrkA is activated in many malignancies including breast, ovarian, prostate, and thyroid carcinomas (8-13). Research studies suggest that expression of TrkA in neuroblastomas may be a good prognostic marker as TrkA signals growth arrest and differentiation of cells originating from the neural crest (10).

Product Categories/Family for anti-NTRK2 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 2497560
• NCBI GeneID: 4915
• UniProt Accession #: Q16620; Q16675; Q59GJ1; Q8WXJ5; Q8WXJ6; Q8WXJ7; B1ANZ4; B4DFV9
• Molecular Weight: 822
• NCBI Official Full Name: BDNF/NT-3 growth factors receptor
• NCBI Official Synonym Full Names: neurotrophic tyrosine kinase, receptor, type 2
• NCBI Official Symbol: NTRK2
• NCBI Official Synonym Symbols: TRKB; trk-B; GP145-TrkB
• NCBI Protein Information: BDNF/NT-3 growth factors receptor; tyrosine kinase receptor B; tropomyosin-related kinase B; BDNF-tropomyosine receptor kinase B
• UniProt Protein Name: BDNF/NT-3 growth factors receptor
• Protein Family: BDNF/NT-3 growth factors receptor
• UniProt Gene Name: NTRK2
• UniProt Synonym Gene Names: TRKB; Trk-B
• UniProt Entry Name: NTRK2_HUMAN

NCBI Description

This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

TrkB: a receptor tyrosine kinase of the Trk family. Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3, -4 and -5 but not nerve growth factor (NGF). Involved in the development and maintenance of the nervous system. Three splice variant isoforms have been described.

Protein type: Membrane protein, integral; Kinase, protein; Protein kinase, TK; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; Trk family

Chromosomal Location of Human Ortholog: 9q22.1

Cellular Component: cell surface; rough endoplasmic reticulum; integral to plasma membrane; dendritic spine; terminal button; excitatory synapse; perikaryon; cytosol; postsynaptic membrane; growth cone; presynaptic active zone; endosome membrane; receptor complex

Molecular Function: brain-derived neurotrophic factor receptor activity; neurotrophin binding; protein homodimerization activity; ephrin receptor binding; brain-derived neurotrophic factor binding; ATP binding

Biological Process: circadian rhythm; mechanoreceptor differentiation; peptidyl-tyrosine phosphorylation; retinal rod cell development; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; neuron migration; regulation of dendrite development; regulation of neurotransmitter secretion; positive regulation of synaptic transmission, glutamatergic; neuron differentiation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; oligodendrocyte differentiation; feeding behavior; negative regulation of neuron apoptosis; inflammatory response; vasculogenesis; neuromuscular junction development; central nervous system neuron development; aging; response to light stimulus; brain-derived neurotrophic factor receptor signaling pathway; adenylate cyclase activation; regulation of protein kinase B signaling cascade; learning; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; peripheral nervous system neuron development; long-term memory; glutamate secretion; positive regulation of axonogenesis; cerebral cortex development; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Obesity, Hyperphagia, And Developmental Delay