Rabbit anti-Mouse, Human Ntrk2 Polyclonal Antibody | anti-Ntrk2 antibody

Ntrk2, ID (Ntrk2, Trkb, BDNF/NT-3 growth factors receptor, GP145-TrkB/GP95-TrkB, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase) (FITC)

Cat. Num. AAA6327010

• Gene Names: Ntrk2; Tkrb; trkB; trk-B; GP145-TrkB/GP95-TrkB
• Reactivity: Mouse, Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Ntrk2; Polyclonal Antibody; ID (Ntrk2; Trkb; BDNF/NT-3 growth factors receptor; GP145-TrkB/GP95-TrkB; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase) (FITC); TrkB tyrosine kinase; anti-Ntrk2 antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Ntrk2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-Ntrk2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within aa342-372 amino acids from the Central region of Mouse Ntrk2 (NP_001020245.1; NP_032771.1).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse NIH/3T3 cell line (35ug per lane), using MBS6007519 (1:1000/lane). A goat anti-rabbit IgG H&L(HRP) (1:5000) was used as the secondary antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue usingMBS6007519, followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6007519 for immunohistochemistry.)

Related Product Information for anti-Ntrk2 antibody

Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.

Product Categories/Family for anti-Ntrk2 antibody

Antibodies; Protein Kinases

References

1. Xu, X., et al. Proc. Natl. Acad. Sci. U.S.A. 107(44):19126-19131(2010). 2. Lobo, M.K., et al. Science 330(6002):385-390(2010). 3. Gascon, E., et al. J. Neurosci. 30(37):12414-12423(2010). 4. Nikoletopoulou, V., et al. Nature 467(7311):59-63(2010). 5. Ohnishi, H., et al. J. Neurosci. 30(31):10472-10483(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 68215970
• NCBI GeneID: 18212
• NCBI Accession #: NP_001020245.1; NP_032771.1
• NCBI GenBank Nucleotide #: NM_001025074.2
• UniProt Accession #: P15209; Q3TUF9; Q80WU0; Q91XJ9
• Molecular Weight: 83,744 Da
• NCBI Official Full Name: BDNF/NT-3 growth factors receptor isoform a
• NCBI Official Synonym Full Names: neurotrophic tyrosine kinase, receptor, type 2
• NCBI Official Symbol: Ntrk2
• NCBI Official Synonym Symbols: Tkrb; trkB; trk-B; GP145-TrkB/GP95-TrkB
• NCBI Protein Information: BDNF/NT-3 growth factors receptor; trkB tyrosine kinase; neurotrophic tyrosine receptor kinase type 2
• UniProt Protein Name: BDNF/NT-3 growth factors receptor
• Protein Family: BDNF/NT-3 growth factors receptor
• UniProt Gene Name: Ntrk2
• UniProt Synonym Gene Names: Trkb; Trk-B
• UniProt Entry Name: NTRK2_MOUSE

Uniprot Description

TrkB: a receptor tyrosine kinase of the Trk family. Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3, -4 and -5 but not nerve growth factor (NGF). Involved in the development and maintenance of the nervous system. Three splice variant isoforms have been described.

Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Membrane protein, integral; Protein kinase, TK; Kinase, protein; TK group; Trk family

Cellular Component: cell surface; rough endoplasmic reticulum; integral to plasma membrane; cell; dendrite; postsynaptic density; integral to membrane; dendritic spine; terminal button; excitatory synapse; perikaryon; cytosol; postsynaptic membrane; growth cone; cell soma; membrane; axon; cytoplasm; plasma membrane; presynaptic active zone; intracellular; nerve terminal; receptor complex; endosome

Molecular Function: transferase activity; brain-derived neurotrophic factor receptor activity; neurotrophin binding; protein binding; protein homodimerization activity; ephrin receptor binding; brain-derived neurotrophic factor binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; transmembrane receptor protein tyrosine kinase activity; neurotrophin receptor activity; ATP binding; protein kinase activity

Biological Process: mechanoreceptor differentiation; peptidyl-tyrosine phosphorylation; retinal rod cell development; protein amino acid autophosphorylation; multicellular organismal development; regulation of neurotransmitter secretion; regulation of dendrite development; neuron migration; positive regulation of synaptic transmission, glutamatergic; protein amino acid phosphorylation; neuron differentiation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; oligodendrocyte differentiation; feeding behavior; negative regulation of neuron apoptosis; regulation of metabolic process; vasculogenesis; cell differentiation; neuromuscular junction development; central nervous system neuron development; nervous system development; regulation of GTPase activity; brain-derived neurotrophic factor receptor signaling pathway; regulation of protein kinase B signaling cascade; learning; positive regulation of peptidyl-serine phosphorylation; peripheral nervous system neuron development; positive regulation of phosphoinositide 3-kinase cascade; glutamate secretion; long-term memory; regulation of MAPKKK cascade; positive regulation of axonogenesis; cerebral cortex development; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway