Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Angiotensin II Receptor 2 (AT2R) Standard Curve.)
Human Angiotensin II Receptor 2 (AT2R) ELISA Kit | AT2R elisa kit
Human Angiotensin II Receptor 2 (AT2R) ELISA Kit
Gene Names
AGTR2; AT2; ATGR2; MRX88
Reactivity
Human
Synonyms
Angiotensin II Receptor 2 (AT2R); Human Angiotensin II Receptor 2 (AT2R) ELISA Kit; AGTR2; AT2; ATGR2; MRX88; angiotensin receptor 2; AT2R elisa kit
Reactivity
Human
Specificity
Human Angiotensin II Receptor 2 (AT2R) ELISA Kit has high sensitivity and excellent specificity for detection of Human AGTR2. No significant cross-reactivity or interference between Human AGTR2 and analogues was observed.
Sequence Length
363
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Angiotensin II Receptor 2 (AT2R) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Angiotensin II Receptor 2 (AT2R) Standard Curve.)
Related Product Information for AT2R elisa kit
Description: This Human Angiotensin II Receptor 2 (AT2R) ELISA Kit employs a two-site sandwich ELISA to quantitate AGTR2 in samples. An antibody specific for AGTR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AGTR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGTR2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGTR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Angiotensin II is a potent pressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors termed AT1 and AT2. AGTR2 belongs to a family 1 of G protein-coupled receptors. It is an integral membrane protein. It plays a role in the central nervous system and cardiovascular functions that are mediated by the renin-angiotensin system. This receptor mediates programmed cell death (apoptosis). In adults, it is highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. It is highly expressed in fetal kidney and intestine. The human AGTR2 gene is composed of three exons and spans at least 5 kb.
Background: Angiotensin II is a potent pressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors termed AT1 and AT2. AGTR2 belongs to a family 1 of G protein-coupled receptors. It is an integral membrane protein. It plays a role in the central nervous system and cardiovascular functions that are mediated by the renin-angiotensin system. This receptor mediates programmed cell death (apoptosis). In adults, it is highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. It is highly expressed in fetal kidney and intestine. The human AGTR2 gene is composed of three exons and spans at least 5 kb.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the G-protein coupled receptor 1 family, and functions as a receptor for angiotensin II. It is an intergral membrane protein that is highly expressed in fetus, but scantily in adult tissues, except brain, adrenal medulla, and atretic ovary. This receptor has been shown to mediate programmed cell death and this apoptotic function may play an important role in developmental biology and pathophysiology. Mutations in this gene are been associated with X-linked cognitive disability. [provided by RefSeq, Jan 2010]
Research Articles on AT2R
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Product Notes
The Human AT2R (Catalog #AAA9714635) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714635 ELISA Kit recognizes Human AT2R. It is sometimes possible for the material contained within the vial of "Angiotensin II Receptor 2 (AT2R), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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