Rabbit anti-Human NRG3 Polyclonal Antibody | anti-NRG3 antibody

NRG3, ID (NRG3, Pro-neuregulin-3, membrane-bound isoform, Neuregulin-3) (APC)

Cat. Num. AAA6326777

• Gene Names: NRG3; HRG3; pro-NRG3
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: NRG3; Polyclonal Antibody; ID (NRG3; Pro-neuregulin-3; membrane-bound isoform; Neuregulin-3) (APC); Neuregulin-3; anti-NRG3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-NRG3 antibody: Immunohistochemistry (IHC), FLISA
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NRG3 antibody is generated from rabbits immunized with a KLH conjugated Synthetic peptide selected from the center region of human NRG3.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NRG3 Antibody (Center) western blot analysis in human blood plasma tissue lysates (35ug/lane).This demonstrates the NRG3 antibody detected the NRG3 protein (arrow).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-NRG3 antibody

NRG3, which belongs to the neuregulin family, is a direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. NRG3 does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. The protein exists as an type I membrane protein and as a proteolytically released soluble growth factor form. The membrane-bound form does not appear to be active. NRG3 is highly expressed in most regions of the brain with the exception of corpus callosum, and is expressed at lower level in testis. It is not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes. The NRG3 cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing may involve initial intracellular domain dimerization.

Product Categories/Family for anti-NRG3 antibody

Antibodies; Membrane Proteins

References

Zhang, D., et al., Proc. Natl. Acad. Sci. U.S.A. 94(18):9562-9567 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 66730481
• NCBI GeneID: 10718
• NCBI Accession #: NP_001010848.2
• NCBI GenBank Nucleotide #: NM_001010848.3
• UniProt Accession #: P56975; Q0PEH2; Q5VYH3; A4D7U1
• Molecular Weight: 75,031 Da
• NCBI Official Full Name: pro-neuregulin-3, membrane-bound isoform isoform 1
• NCBI Official Synonym Full Names: neuregulin 3
• NCBI Official Symbol: NRG3
• NCBI Official Synonym Symbols: HRG3; pro-NRG3
• NCBI Protein Information: pro-neuregulin-3, membrane-bound isoform; neuregulin 3 variant 1; neuregulin 3 variant 3; neuregulin 3 variant 4; neuregulin 3 variant 5; neuregulin 3 variant 6; neuregulin 3 variant 7; neuregulin 3 variant 8; neuregulin 3 variant 9; neuregulin 3 variant
• UniProt Protein Name: Pro-neuregulin-3, membrane-bound isoform
• Protein Family: Pro-neuregulin
• UniProt Gene Name: NRG3
• UniProt Synonym Gene Names: Pro-NRG3; NRG-3
• UniProt Entry Name: NRG3_HUMAN

NCBI Description

This gene is a member of the neuregulin gene family. This gene family encodes ligands for the transmembrane tyrosine kinase receptors ERBB3 and ERBB4 - members of the epidermal growth factor receptor family. Ligand binding activates intracellular signaling cascades and the induction of cellular responses including proliferation, migration, differentiation, and survival or apoptosis. This gene encodes neuregulin 3 (NRG3). NRG3 has been shown to activate the tyrosine phosphorylation of its cognate receptor, ERBB4, and is thought to influence neuroblast proliferation, migration and differentiation by signalling through ERBB4. NRG3 also promotes mammary differentiation during embryogenesis. Linkage studies have implicated this gene as a susceptibility locus for schizophrenia and schizoaffective disorder. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but their biological validity has not been verified.[provided by RefSeq, Sep 2009]

Uniprot Description

NRG3: Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes. Belongs to the neuregulin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligand, receptor tyrosine kinase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q23.1

Cellular Component: extracellular space; integral to plasma membrane; extracellular region

Molecular Function: transmembrane receptor protein tyrosine kinase activator activity; growth factor activity; receptor tyrosine kinase binding; receptor binding

Biological Process: transmembrane receptor protein tyrosine kinase activation (dimerization); organ development; regulation of cell growth; pattern specification process