Western Blot (WB)
((1ug/ml) staining of HepG2 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat NR5A2/LRH1 Polyclonal Antibody | anti-NR5A2 antibody
Goat anti-NR5A2/LRH1 Antibody
Western Blot: Approx 55kDa band observed in nuclear lysates of cell lines HeLa and HepG2, and approx 60kDa band observed in Pig Ovary lysates (calculated MW of 56.5kDa according to Human NP_003813.1 and Pig AFD98842.1). Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((1ug/ml) staining of HepG2 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of HepG2 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Pig Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Pig Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
Uniprot Description
NR5A2: Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. Belongs to the nuclear hormone receptor family. NR5 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Nuclear receptor; Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: cytoplasm; nucleoplasm; nucleus
Molecular Function: chromatin binding; DNA binding; ligand-dependent nuclear receptor activity; phospholipid binding; protein binding; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor activity; zinc ion binding
Biological Process: bile acid metabolic process; cholesterol homeostasis; embryonic development; epithelial cell differentiation; homeostatic process; hormone-mediated signaling; intracellular receptor-mediated signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of viral genome replication; regulation of cell proliferation; regulation of transcription, DNA-dependent; steroid hormone mediated signaling; tissue development; transcription initiation from RNA polymerase II promoter
Research Articles on NR5A2
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Product Notes
The NR5A2 nr5a2 (Catalog #AAA423452) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-NR5A2/LRH1 Antibody reacts with Tested: Human, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's NR5A2/LRH1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 55kDa band observed in nuclear lysates of cell lines HeLa and HepG2, and approx 60kDa band observed in Pig Ovary lysates (calculated MW of 56.5kDa according to Human NP_003813.1 and Pig AFD98842.1). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the NR5A2 nr5a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TDYDRSPFVT SPIS. It is sometimes possible for the material contained within the vial of "NR5A2/LRH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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