Rabbit anti-Human NR5A1 Polyclonal Antibody | anti-NR5A1 antibody

NR5A1, NT (NR5A1, AD4BP, FTZF1, SF1, Steroidogenic factor 1, Adrenal 4-binding protein, Fushi tarazu factor homolog 1, Nuclear receptor subfamily 5 group A member 1, Steroid hormone receptor Ad4BP) (FITC)

Cat. Num. AAA6326680

• Gene Names: NR5A1; ELP; SF1; FTZ1; POF7; SF-1; AD4BP; FTZF1; SPGF8; SRXY3
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NR5A1; Polyclonal Antibody; NT (NR5A1; AD4BP; FTZF1; SF1; Steroidogenic factor 1; Adrenal 4-binding protein; Fushi tarazu factor homolog 1; Nuclear receptor subfamily 5 group A member 1; Steroid hormone receptor Ad4BP) (FITC); Steroid hormone receptor Ad4BP; anti-NR5A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NR5A1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NR5A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-100 amino acids from the N-terminal region of human NR5A1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NR5A1 Antibody (N-term) in HepG2 cell line lysates (35ug/lane).NR5A1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(NR5A1 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NR5A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(NR5A1 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-NR5A1 antibody

NR5A1 is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this protein are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect.

Product Categories/Family for anti-NR5A1 antibody

Antibodies; Binding Proteins

References

Almeida, M.Q., et al. J. Clin. Endocrinol. Metab. 95(3):1458-1462(2010). Ramayya, M.S., et al. J. Steroid Biochem. Mol. Biol. 119 (1-2), 14-25 (2010). Catalano, S., et al. J. Biol. Chem. 285(8):5581-5593(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 20070193
• NCBI GeneID: 2516
• NCBI Accession #: NP_004950.2
• NCBI GenBank Nucleotide #: NM_004959.4
• UniProt Accession #: Q13285; O15196; Q5T6F5
• Molecular Weight: 51,636 Da
• NCBI Official Full Name: steroidogenic factor 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 5, group A, member 1
• NCBI Official Symbol: NR5A1
• NCBI Official Synonym Symbols: ELP; SF1; FTZ1; POF7; SF-1; AD4BP; FTZF1; SPGF8; SRXY3
• NCBI Protein Information: steroidogenic factor 1; STF-1; nuclear receptor AdBP4; steroidogenic factor-1; adrenal 4 binding protein; adrenal 4-binding protein; fushi tarazu factor homolog 1; steroid hormone receptor Ad4BP; steroidogenic factor 1 nuclear receptor
• UniProt Protein Name: Steroidogenic factor 1
• Protein Family: Steroidogenic factor
• UniProt Gene Name: NR5A1
• UniProt Synonym Gene Names: AD4BP; FTZF1; SF1; SF-1; STF-1
• UniProt Entry Name: STF1_HUMAN

NCBI Description

The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect. [provided by RefSeq, Jul 2008]

Uniprot Description

STF-1: a nuclear hormone receptor of the NR5 subfamily. Essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P-450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the Muellerian inhibiting substance (AMH) gene as well as the AHCH and STAR genes.

Protein type: Nuclear receptor

Chromosomal Location of Human Ortholog: 9q33

Cellular Component: nucleoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; ligand-dependent nuclear receptor activity; enzyme binding; DNA binding; zinc ion binding; sequence-specific DNA binding; transcription coactivator activity; double-stranded DNA binding; steroid hormone receptor activity; phospholipid binding; chromatin binding

Biological Process: transcription initiation from RNA polymerase II promoter; tissue development; intracellular receptor-mediated signaling pathway; adrenal gland development; primary sex determination; male gonad development; multicellular organismal aging; regulation of steroid biosynthetic process; cell-cell signaling; luteinization; hormone metabolic process; gene expression; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; maintenance of protein localization in nucleus; cell differentiation

Disease: Premature Ovarian Failure 7; 46,xy Sex Reversal 3; Spermatogenic Failure 8