Rabbit NR5A1 Polyclonal Antibody | anti-NR5A1 antibody

NR5A1 Rabbit Polyclonal

Cat. Num. AAA7602080

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: NR5A1; Polyclonal Antibody; NR5A1 Rabbit Polyclonal; AD4BP; Adrenal 4 binding protein; ELP; FTZl; FTZFl; Fushi tarazu factor homolog 1; SF 1; SFI; SF-I; Steroid hormone receptor Ad4BP; Steroidogenic factor 1; STF I; anti-NR5A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, Mouse, Rat; other species are not tested. Please decide the specificity by homology.
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid

• Applicable Applications for anti-NR5A1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:5000; IP: 1:200-1:1OOO; IHC: 1:20-1:200
• Immunogen: Nuclear receptor subfamily 5, group A, member 1
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver using MBS7602080 (NR5A1 antibody) at dilution of 1:100)

Immunoprecipitation (IP)

(IP Result of anti-NR5A1 (IP: MBS7602080, 4ug; Detection: MBS7602080 1:300) with A2780 cells lysate 960ug.)

Western Blot (WB)

(mouse ovary tissue were subjected to SDS PAGE followed by western blot with MBS7602080 (NR5A1 antibody) at dilution of 1:1000)

Related Product Information for anti-NR5A1 antibody

Steroidogenic factor-l (SF-l,STF-l), also known as NR5A1, regulates multiple genes involved in the adrenal and gonadal development and in the biosynthesis of a variety of hormones, including adrenal and gonadal steroids, anti-Mullerian hormone (AMH), and gonadotropins. SF-l belongs to the fushi tarazu factor-l (FTZ-F 1) subfamily of orphan nuclear receptors. Initially identified as a tissue-specific transcriptional regulator of cytochrome P450 steroid hydroxylases, research studies of both global and tissue-specific knockout mice have demonstrated that SF -1 is required for the development of adrenal glands, gonads, ventromedial hypothalamus, and for the proper functioning of pituitary gonadotropes. Indeed, humans with lliutations that render SF -1 transcriptionally inactive can present with testicular failure, ovarian failure, and adrenal insufficiency. Furthermore, dysregulation of SF -1 has been linked to diseases such as endometriosis and adrenocortical carcinoma. Like other nuclear hormone receptors, SF-1 has a modular domain structure composed of an N-terminal zinc finger DNA-binding domain, a ligand-binding domain, a C-terminal AF-2 activation domain, and a hinge region with A}7-1-like activation activity. SF-l also contains a fushi tarazu factor 1 box, which functions as an accessory DNA binding domain. SF-l is primarily phosphorylated at Ser203, which is thought to enhance its transcriptional activity by promoting complex formation with transcriptional cofactors. In addition to phosphorylation at Ser203, SF-l is subject to SUMO conjugation and acetylation at E-amino groups of target lysine residues. Whereas SUMOylation represses SF-1 function, acetylation enhances its transcriptional activity.In the adult ovary, SF -1 localizes to theca/interstitial cells.

NCBI and Uniprot Product Information

• UniProt Accession #: Q13285
• Molecular Weight: 52kDa
• Protein Family: Steroidogenic factor

Uniprot Description

Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phosphatidylcholine (). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.