Rabbit NR5A1 / SF1 Polyclonal Antibody | anti-NR5A1 antibody

Rabbit Polyclonal to Human NR5A1 / SF1

Cat. Num. AAA241424

• Gene Names: NR5A1; ELP; SF1; FTZ1; POF7; SF-1; AD4BP; FTZF1; SPGF8; SRXY3
• Reactivity: Human; Predicted Reactivity: Gorilla, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Rabbit (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: NR5A1 / SF1; Polyclonal Antibody; Rabbit Polyclonal to Human NR5A1 / SF1; Anti-NR5A1 / SF1 Antibody (C-Terminus) IHC-plus; NR5A1; AD4BP; Adrenal 4 binding protein; Adrenal 4-binding protein; ELP; Steroidogenic factor-1; SRXY3; Steroidogenic factor 1; Nuclear receptor AdBP4; Nuclear receptor elp; POF7; SF-1; SPGF8; Steroid hormone receptor Ad4BP; STF-1; FTZ1; FTZF1; Human NR5A1; SF1; anti-NR5A1 antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Gorilla, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Rabbit (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human NR5A1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 0.7 mg/ml (varies by lot)
• Sequence Length: 461

• Applicable Applications for anti-NR5A1 antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (3.2 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic 17 amino acid peptide from near C-terminus of human NR5A1. Percent identity with other species by BLAST analysis: Human (100%); Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Bovine, Hamster, Elephant, Rabbit, Opossum (94%).
• Immunogen Type: Synthetic peptide
• Immunogen: NR5A1 / SF1 antibody was raised against synthetic 17 amino acid peptide from near C-terminus of human NR5A1. Percent identity with other species by BLAST analysis: Human (100%); Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Bovine, Hamster, Elephant, Rabbit, Opossum (94%); Panda, Horse, Pig, Turkey, Chicken (88%); Platypus (82%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-NR5A1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-NR5A1 antibody

Steroidogenic factor 1 (SF-1), a NR5 Fushi Tarazu-Like Receptor, has been shown to affect sexual differentiation, steroid metabolism, and neural development. SF-1 binds as a monomer and regulates all steroidogenic p450 genes, Mullerian inhibiting substance, the gonadotrophins, the ACTH receptor, oxytocin, nuclear receptor DAX-1, and several other genes.

Product Categories/Family for anti-NR5A1 antibody

Family: NHR; Subfamily: NR5 Fushi tarazu-like

NCBI and Uniprot Product Information

• NCBI GI #: 20070193
• NCBI GeneID: 2516
• NCBI Accession #: NP_004950.2
• NCBI GenBank Nucleotide #: NM_004959.4
• UniProt Accession #: Q13285; O15196; Q5T6F5
• Molecular Weight: 51,636 Da
• NCBI Official Full Name: steroidogenic factor 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 5, group A, member 1
• NCBI Official Symbol: NR5A1
• NCBI Official Synonym Symbols: ELP; SF1; FTZ1; POF7; SF-1; AD4BP; FTZF1; SPGF8; SRXY3
• NCBI Protein Information: steroidogenic factor 1; STF-1; nuclear receptor AdBP4; steroidogenic factor-1; adrenal 4 binding protein; adrenal 4-binding protein; fushi tarazu factor homolog 1; steroid hormone receptor Ad4BP; steroidogenic factor 1 nuclear receptor
• UniProt Protein Name: Steroidogenic factor 1
• Protein Family: Steroidogenic factor
• UniProt Gene Name: NR5A1
• UniProt Synonym Gene Names: AD4BP; FTZF1; SF1; SF-1; STF-1
• UniProt Entry Name: STF1_HUMAN

NCBI Description

The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect. [provided by RefSeq, Jul 2008]

Uniprot Description

STF-1: a nuclear hormone receptor of the NR5 subfamily. Essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P-450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the Muellerian inhibiting substance (AMH) gene as well as the AHCH and STAR genes.

Protein type: Nuclear receptor

Chromosomal Location of Human Ortholog: 9q33

Cellular Component: nucleoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; ligand-dependent nuclear receptor activity; enzyme binding; DNA binding; zinc ion binding; sequence-specific DNA binding; double-stranded DNA binding; transcription coactivator activity; phospholipid binding; steroid hormone receptor activity; chromatin binding

Biological Process: transcription initiation from RNA polymerase II promoter; tissue development; intracellular receptor-mediated signaling pathway; adrenal gland development; primary sex determination; male gonad development; multicellular organismal aging; regulation of steroid biosynthetic process; cell-cell signaling; luteinization; hormone metabolic process; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; gene expression; maintenance of protein localization in nucleus; cell differentiation

Disease: Premature Ovarian Failure 7; 46,xy Sex Reversal 3; Spermatogenic Failure 8