Rabbit anti-Human NR4A2 Polyclonal Antibody | anti-NR4A2 antibody

NR4A2, NT (Nuclear Receptor Subfamily 4 Group A Member 2, Orphan Nuclear Receptor NURR1, NURR1, TINUR, NURR1, Immediate-early Response Protein NOT, NOT, Transcriptionally Inducible Nuclear Receptor) (AP)

Cat. Num. AAA6490694

• Gene Names: NR4A2; NOT; RNR1; HZF-3; NURR1; TINUR
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: NR4A2; Polyclonal Antibody; NT (Nuclear Receptor Subfamily 4 Group A Member 2; Orphan Nuclear Receptor NURR1; NURR1; TINUR; Immediate-early Response Protein NOT; NOT; Transcriptionally Inducible Nuclear Receptor) (AP); anti-NR4A2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NR4A2.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-NR4A2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 13-42 in the N-terminal region of human NR4A2, UniProt Accession #P43354.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse brain tissue lysate usingMBS623814. This demonstrates MBS623814 detected the NR4A2 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in Hela cell line lysates (35ug/lane) usingMBS623814. This demonstrates that MBS623814 detected the NR4A2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS623814 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS623814 for immunohistochemistry.)

Related Product Information for anti-NR4A2 antibody

Parkinson's disease (PD) is a multifactorial disease that appears to arise from the effects of both genetic and environmental influences. The known genetic factors include multiple genes that have been identified in related parkinsonian syndromes, as well as alpha-synuclein. Genes associated with either PD or Parkinson-related disorders include parkin, DJ-1, ubiquitin C-terminal hydrolase isozyme L1 (UCH-L1), nuclear receptor-related factor 1 (NURR1), and alpha-synuclein. Nurr1 is a transcription factor that is expressed in the embryonic ventral midbrain and is critical for the development of dopamine (DA) neurons. It belongs to the conserved family of nuclear receptors but lacks an identified ligand and is therefore referred to as an orphan receptor. RXR ligands can promote the survival of DA neurons via a process that depends on Nurr1-RXR heterodimers. In developing DA cells, Nurr1 is required for the expression of several genes important for DA synthesis and function. Nurr1 is also important for the maintenance of adult DA neurons.

Product Categories/Family for anti-NR4A2 antibody

Antibodies; Receptors

References

1. Perlmann T, et al. Cell Tissue Res. 318(1):45-52 (2004). 2. Hsu HC, et al. Curr Drug Targets Inflamm Allergy. 3(4):413-23 (2004). 3. Wallen-Mackenzie A, et al. Genes Dev. 17(24):3036-47 (2003). 4. Ichinose, H., et al. Gene 230(2), 233-239 (1999). 5. Okabe, T., et al. J. Immunol. 154(8), 3871-3879 (1995) 6.Mages, H.W., et al. Mol. Endocrinol. 8(11), 1583-1591 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 187423888
• NCBI GeneID: 4929
• NCBI Accession #: NP_006177.1
• NCBI GenBank Nucleotide #: NM_006186
• UniProt Accession #: P43354; Q16311; Q53RZ2; Q6NXU0
• Molecular Weight: 59,809 Da
• NCBI Official Full Name: Homo sapiens nuclear receptor subfamily 4, group A, member 2 (NR4A2), mRNA
• NCBI Official Synonym Full Names: nuclear receptor subfamily 4, group A, member 2
• NCBI Official Symbol: NR4A2
• NCBI Official Synonym Symbols: NOT; RNR1; HZF-3; NURR1; TINUR
• NCBI Protein Information: nuclear receptor subfamily 4 group A member 2; NGFI-B/nur77 beta-type transcription factor homolog; T-cell nuclear receptor NOT; immediate-early response protein NOT; intermediate-early receptor protein; nuclear receptor related 1; nur related protein-1,
• UniProt Protein Name: Nuclear receptor subfamily 4 group A member 2
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR4A2
• UniProt Synonym Gene Names: NOT; NURR1; TINUR
• UniProt Entry Name: NR4A2_HUMAN

NCBI Description

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. The encoded protein may act as a transcription factor. Mutations in this gene have been associated with disorders related to dopaminergic dysfunction, including Parkinson disease, schizophernia, and manic depression. Misregulation of this gene may be associated with rheumatoid arthritis. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons ().