Rabbit anti-Human NR4A1 Polyclonal Antibody | anti-NR4A1 antibody

NR4A1, Phosphorylated (Ser351) (Nuclear Receptor Subfamily 4 Group A Member 1, Orphan Nuclear Receptor TR3, Orphan Nuclear Receptor HMR, Early Response Protein NAK1, Testicular Receptor 3, ST-59, Nur77, GFRP1, HMR, NAK1) (FITC)

Cat. Num. AAA6489287

• Gene Names: NR4A1; HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77
• Reactivity: Human
• Applications: Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NR4A1; Polyclonal Antibody; Phosphorylated (Ser351) (Nuclear Receptor Subfamily 4 Group A Member 1; Orphan Nuclear Receptor TR3; Orphan Nuclear Receptor HMR; Early Response Protein NAK1; Testicular Receptor 3; ST-59; Nur77; GFRP1; HMR; NAK1) (FITC); anti-NR4A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NR4A1 when phosphorylated at Ser351.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NR4A1 antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Dot Blot: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic phosphopeptide mapping to a fragment of amino acid residues surrounding S351 of human NR4A1, UniProt Accession #P22736. Species sequence homology: mouse, rat
• Conjugate: FITC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis on nitrocellulose membrane usingMBS627516. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.)

Product Categories/Family for anti-NR4A1 antibody

Antibodies; Receptors

References

1. Lu, L., et al., J. Clin. Endocrinol. Metab. 89(8):4113-4118 (2004). 2. Castro-Obregon, S., et al., J. Biol. Chem. 279(17):17543-17553 (2004). 3. Lin, B., et al., Cell 116(4):527-540 (2004). 4. Choi, J.W., et al., Cancer Res. 64(1):35-39 (2004). 5. Ye, X., et al., Int. J. Biochem. Cell Biol. 36(1):98-113 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 21361342
• NCBI GeneID: 3164
• NCBI Accession #: NP_002126
• NCBI GenBank Nucleotide #: NM_002135.4
• UniProt Accession #: P22736; Q15627; Q53Y00; Q6IBU8; B4DML7
• Molecular Weight: 33,754 Da
• NCBI Official Full Name: nuclear receptor subfamily 4 group A member 1 isoform 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 4, group A, member 1
• NCBI Official Symbol: NR4A1
• NCBI Official Synonym Symbols: HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77
• NCBI Protein Information: nuclear receptor subfamily 4 group A member 1
• UniProt Protein Name: Nuclear receptor subfamily 4 group A member 1
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR4A1
• UniProt Synonym Gene Names: GFRP1; HMR; NAK1; Nur77
• UniProt Entry Name: NR4A1_HUMAN

NCBI Description

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

Nur77: an orphan nuclear receptor and immediate-early gene that regulates cellular proliferation, apoptosis, inflammation, and glucose metabolism. Induced by exercise in muscle and is a functional regulator of glucose metabolism in skeletal muscle. Its level decreases in the muscle of obese insulin-resistant men. Acts concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. A mediator of TCR-directed thymocyte apoptosis. TCR-signaling induces a FAIM/Akt/Nur77 signaling pathway that is critical for modulating apoptosis in developing thymocytes. A physiological substrate of the MEK-ERK-RSK cascade that modulates nuclear export and intracellular translocation during T cell death. Binds DNA as a monomer. Interacts with GADD45GIP1. Overexpression of Nur77 induces the expression of both p300 and HDAC1. Acetylation by p300 and HDAC1 may regulate the rapid turnover of Nur77 protein.

Protein type: DNA-binding; Apoptosis; Nuclear receptor

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity

Biological Process: epidermal growth factor receptor signaling pathway; transcription initiation from RNA polymerase II promoter; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; intracellular receptor-mediated signaling pathway; nerve growth factor receptor signaling pathway; signal transduction; cell migration during sprouting angiogenesis; innate immune response; positive regulation of transcription from RNA polymerase II promoter; gene expression; steroid hormone mediated signaling; positive regulation of endothelial cell proliferation