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Western Blot (WB) (Western Blot showing NR2F2 antibody used at a concentration of 2.5 ug/ml against MCF7 Cell Lysate)

Rabbit NR2F2 Polyclonal Antibody | anti-NR2F2 antibody

NR2F2 antibody

Gene Names
NR2F2; ARP1; CHTD4; NF-E3; NR2F1; SVP40; COUPTFB; TFCOUP2; COUPTFII
Reactivity
Cow, dog, guinea pig, human, mouse, rat, zebrafish.
Applications
Immunohistochemistry, Western Blot
Synonyms
NR2F2; Polyclonal Antibody; NR2F2 antibody; Polyclonal NR2F2; Anti-NR2F2; ARP1; SVP40; nuclear receptor subfamily 2; COUP-TFII; NRF-2; MGC117452; member 2; TFCOUP2; group F; COUPTFB; NRF 2; anti-NR2F2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Cow, dog, guinea pig, human, mouse, rat, zebrafish.
Clonality
Polyclonal
Form/Format
100ug lyophilzed powder in PBS buffer with 2% sucrose. Reconstitute with 100ul distilled water for a final concentration of 1mg/ml.
Concentration
100ug/lyophilized (varies by lot)
Sequence Length
414
Applicable Applications for anti-NR2F2 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
Optimal conditions to be determined by the end user
Biological Significance
NR2F2, an orphan member of the nuclear hormone receptor superfamily, acts as a transcriptional repressor by antagonizing the functions of other nuclear hormone receptors and by actively silencing transcription. However, in certain contexts, NR2F2 stimulates transcription directly. NR2F2, MyoD and p300 interact in a competitive manner, and that increasing amounts of NR2F2 have the ability to reduce the interaction between myoD and p300 invitro. NR2F2 post-transcriptionally regulates myoD activity/function, and that crosstalk between orphan nuclear receptors and the myogenic bHLH proteins has functional consequences for differentiation.
Cross-Reactivity
Human, Mouse, Rat, Dog, Bovine, Chicken, Zebrafish, African Clawed Frog
Immunogen
synthetic peptide directed toward the N terminal of human NR2F2.
Preparation and Storage
Ships ambient or refrigerated. Upon receiving, store at 4 degree C for short term. Store at -20 degree C for long term.

Western Blot (WB)

(Western Blot showing NR2F2 antibody used at a concentration of 2.5 ug/ml against MCF7 Cell Lysate)

Western Blot (WB) (Western Blot showing NR2F2 antibody used at a concentration of 2.5 ug/ml against MCF7 Cell Lysate)

Immunohistochemistry (IHC)

(NR2F2 antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml to stain Epithelial cells of renal tubule (arrows) in Human Kidney. Magnification is at 400X)

Immunohistochemistry (IHC) (NR2F2 antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml to stain Epithelial cells of renal tubule (arrows) in Human Kidney. Magnification is at 400X)
Related Product Information for anti-NR2F2 antibody
Rabbit polyclonal NR2F2 antibody
Product Categories/Family for anti-NR2F2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,163 Da
NCBI Official Full Name
COUP transcription factor 2 isoform a
NCBI Official Synonym Full Names
nuclear receptor subfamily 2, group F, member 2
NCBI Official Symbol
NR2F2
NCBI Official Synonym Symbols
ARP1; CHTD4; NF-E3; NR2F1; SVP40; COUPTFB; TFCOUP2; COUPTFII
NCBI Protein Information
COUP transcription factor 2
UniProt Protein Name
COUP transcription factor 2
Protein Family
UniProt Gene Name
NR2F2
UniProt Synonym Gene Names
ARP1; TFCOUP2; COUP-TF2; ARP-1; COUP-TF II
UniProt Entry Name
COT2_HUMAN

NCBI Description

This gene encodes a member of the steroid thyroid hormone superfamily of nuclear receptors. The encoded protein is a ligand inducible transcription factor that is involved in the regulation of many different genes. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

NR2F2: Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A. Interacts with SQSTM1. Binds DNA as a dimer; homodimer or heterodimer with NR2F6. Interacts with NCOA1, NCOA2, NCOA3 and PPARGC1A. Interacts with ZFPM2. Ubiquitous. Belongs to the nuclear hormone receptor family. NR2 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor; DNA-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q26

Cellular Component: nucleus

Molecular Function: ligand-dependent nuclear receptor activity; protein binding; protein homodimerization activity; retinoic acid binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription corepressor activity; transcription factor activity

Biological Process: limb development; skeletal muscle development; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; negative regulation of cyclin-dependent protein kinase activity; neuron migration; negative regulation of transcription from RNA polymerase II promoter; signal transduction; response to estradiol stimulus; regulation of transcription from RNA polymerase II promoter; anterior/posterior pattern formation; fertilization; forebrain development; negative regulation of endothelial cell proliferation; blood vessel morphogenesis; steroid hormone mediated signaling; lipid metabolic process; negative regulation of transcription, DNA-dependent; radial pattern formation; maternal placenta development

Disease: Congenital Heart Defects, Multiple Types, 4

Research Articles on NR2F2

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Product Notes

The NR2F2 nr2f2 (Catalog #AAA536467) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NR2F2 antibody reacts with Cow, dog, guinea pig, human, mouse, rat, zebrafish. and may cross-react with other species as described in the data sheet. AAA Biotech's NR2F2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Optimal conditions to be determined by the end user. Researchers should empirically determine the suitability of the NR2F2 nr2f2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NR2F2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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