Rabbit anti-Human NR2C1 Polyclonal Antibody | anti-NR2C1 antibody

NR2C1, CT (NR2C1, TR2, Nuclear receptor subfamily 2 group C member 1, Orphan nuclear receptor TR2, Testicular receptor 2) (AP)

Cat. Num. AAA6326534

• Gene Names: NR2C1; TR2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NR2C1; Polyclonal Antibody; CT (NR2C1; TR2; Nuclear receptor subfamily 2 group C member 1; Orphan nuclear receptor TR2; Testicular receptor 2) (AP); Testicular receptor 2; anti-NR2C1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-NR2C1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NR2C1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 515-545 amino acids from the C-terminal region of human NR2C1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NR2C1 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NR2C1 antibody detected the NR2C1 protein (arrow).)

Related Product Information for anti-NR2C1 antibody

This gene encodes a nuclear hormone receptor characterized by a highly conserved DNA binding domain (DBD), a variable hinge region, and a carboxy-terminal ligand binding domain (LBD) that is typical for all members of the steroid/thyroid hormone receptor superfamily. This protein also belongs to a large family of ligand-inducible transcription factors that regulate gene expression by binding to specific DNA sequences within promoters of target genes. Multiple alternatively spliced transcript variants have been described, but the full-length nature of some of these variants has not been determined.

Product Categories/Family for anti-NR2C1 antibody

Antibodies; Receptors

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Lin, Y.L., et al. Biochem. Biophys. Res. Commun. 350(2):430-436(2006). Li, G., et al. Biochem. Biophys. Res. Commun. 310(2):384-390(2003). Mu, X., et al. Prostate 57(2):129-133(2003). Franco, P.J., et al. Mol. Endocrinol. 15(8):1318-1328(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 73808084
• NCBI GeneID: 7181
• NCBI Accession #: NP_001027458.1; NP_001120834.1
• NCBI GenBank Nucleotide #: NM_001032287.2
• UniProt Accession #: P13056; Q15625; Q15626; A8K5K4
• Molecular Weight: 67,315 Da
• NCBI Official Full Name: nuclear receptor subfamily 2 group C member 1 isoform b
• NCBI Official Synonym Full Names: nuclear receptor subfamily 2, group C, member 1
• NCBI Official Symbol: NR2C1
• NCBI Official Synonym Symbols: TR2
• NCBI Protein Information: nuclear receptor subfamily 2 group C member 1; orphan nuclear receptor TR2; TR2 nuclear hormone receptor; nuclear receptor subfamily 2, group C isoform
• UniProt Protein Name: Nuclear receptor subfamily 2 group C member 1
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR2C1
• UniProt Synonym Gene Names: TR2
• UniProt Entry Name: NR2C1_HUMAN

Uniprot Description

NR2C1: Orphan nuclear receptor. Binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. Primarily repressor of a broad range of genes. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Together with NR2C2, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription. Also activator of OCT4 gene expression. May be involved in stem cell proliferation and differentiation. Mediator of retinoic acid- regulated preadipocyte proliferation. Belongs to the nuclear hormone receptor family. NR2 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: nucleoplasm; PML body

Molecular Function: protein binding; protein homodimerization activity; DNA binding; zinc ion binding; sequence-specific DNA binding; histone deacetylase binding; steroid hormone receptor activity; receptor activity; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; positive regulation of retinoic acid receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter