Western Blot (WB)
(Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1472 (Control). The phosphospecificity of this labeling is shown in the second Lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the phospho-Tyr1472 NMDA NR2B antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.)
Rabbit NR2B Polyclonal Antibody | anti-NR2B antibody
Phospho-NR2B antibody
Applications
Western Blot
Purity
Phospho-NR2B antibody was purified by affinity chromatography
Synonyms
NR2B; Polyclonal Antibody; Phospho-NR2B antibody; Polyclonal Phospho-NR2B; anti-Phospho-NR2B; NMDA; Tyr1472; Phospho-NRB-2; Phospho-NRB 2; Phospho-NR2B; anti-NR2B antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Phospho-NR2B antibody was purified by affinity chromatography
Form/Format
Supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol
Applicable Applications for anti-NR2B antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Biological Significance
The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate are designated NMDA receptors. The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer?s, epilepsy and ischemic neuronal cell death. The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS. Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 subunits. Overexpression of the NR2B-subunit of the NMDA Receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. Recent work suggests that phosphorylation of Tyr1472 on NR2B may regulate the functional expression the receptor in LTP and other forms of plasticity.
Cross-Reactivity
Rat, Bovine, Canine, Chicken, Human, Mouse
Immunogen
Phospho-NR2B antibody was raised in Rabbit using Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1472 of NMDA NR2B as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1472 (Control). The phosphospecificity of this labeling is shown in the second Lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the phospho-Tyr1472 NMDA NR2B antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.)
Western Blot (WB)
(Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1472 (Control). The phosphospecificity of this labeling is shown in the second Lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the phospho-Tyr1472 NMDA NR2B antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.)
Related Product Information for anti-NR2B antibody
Rabbit polyclonal Phospho-NR2B antibody
Product Categories/Family for anti-NR2B antibody
Similar Products
Product Notes
The NR2B (Catalog #AAA835048) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NR2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the NR2B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NR2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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