Rabbit NQO2 Polyclonal Antibody | anti-NQO2 antibody

NQO2 Polyclonal Antibody

Cat. Num. AAA3013147

• Gene Names: NQO2; QR2; DHQV; DIA6; NMOR2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: ProA affinity purified
• Synonyms: NQO2; Polyclonal Antibody; NQO2 Polyclonal Antibody; QR2; DHQV; DIA6; EC 1.10.99.2; MGC94180; NAD(P)H dehydrogenase quinone 2; NAD(P)H menadione oxidoreductase 1 dioxin inducible 2; NAD(P)H menadione oxidoreductase 2 dioxin inducible; NMOR2; NQO 2; NRH dehydrogenase [quinone] 2; NRH dehydrogenase; NRH: quinone oxidoreductase 2; OTTHUMP00000015948; OTTHUMP00000015949; OTTHUMP00000015953; Ox 2; Ox2; Quinone; Quinone reductase 2; Ribosyldihydronicotinamide dehydrogenase [quinone]; Ribosyldihydronicotinamide dehydrogenase; anti-NQO2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NQO2 polyclonal antibody detects endogenous levels of NQO2 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 231

• Applicable Applications for anti-NQO2 antibody: Western Blot (WB)
• Application Notes: WB: 1:1,000-1:2,000
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of NQO2 on Hela cells lysates using anti-NQO2 antibody at 1/1,000 dilution.)

Related Product Information for anti-NQO2 antibody

NAD(P)H: quinone oxidoreductase 1 (NQO1) and NRH: quinone oxidoreductase (NQO2) are flavoproteins that catalyze the metabolic detoxification of quinones and their derivatives to hydroquinones. This detoxification process protects cells against quinone-induced oxidative stress, cytotoxicity and mutagenicity. NQO2 is a 231 amino acid protein and is 43 amino acids shorter than NQO1 at its C-terminus. NQO2 is an isozyme of NQO1 and transfers two electrons to a quinone, resulting in the formation of a hydroquinone product. The NQO2 gene is ubiquitously expressed and induced in response to TCDD. NQO2 has a higher level of expression in mouse liver and testis than NQO1, which is highly expressed in the heart. NQO2 has a different cofactor requirement than NQO1 and uses dihydronicotinamide riboside (NRH) rather than NAD(P)H as an electron donor. Unlike NQO1, NQO2 is resistant to typical inhibitors of NQO1 such as dicoumarol, Cibacron blue and phenindonee, but is inhibited by quercetin and benzo(a)pyrene. NQO2 contains a specific metal binding site, which is absent in NQO1 and several cis-elements including SP1 binding sites, CCAAT box, XRE and ARE, which are located at the NQO2 gene promoter.

NCBI and Uniprot Product Information

• NCBI GI #: 317373581
• NCBI GeneID: 4835
• UniProt Accession #: P16083; Q9JI75; Q6AY80
• Molecular Weight: 26kDa
• NCBI Official Full Name: Ribosyldihydronicotinamide dehydrogenase
• NCBI Official Synonym Full Names: N-ribosyldihydronicotinamide:quinone reductase 2
• NCBI Official Symbol: NQO2
• NCBI Official Synonym Symbols: QR2; DHQV; DIA6; NMOR2
• NCBI Protein Information: ribosyldihydronicotinamide dehydrogenase [quinone]
• UniProt Protein Name: Ribosyldihydronicotinamide dehydrogenase [quinone]
• Protein Family: NADH-quinone oxidoreductase
• UniProt Gene Name: NQO2
• UniProt Synonym Gene Names: NMOR2; QR2
• UniProt Entry Name: NQO2_HUMAN

NCBI Description

This gene encodes a member of the thioredoxin family of enzymes. It is a cytosolic and ubiquitously expressed flavoprotein that catalyzes the two-electron reduction of quinone substrates and uses dihydronicotinamide riboside as a reducing coenzyme. Mutations in this gene have been associated with neurodegenerative diseases and several cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

NQO2: The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. Belongs to the NAD(P)H dehydrogenase (quinone) family.

Protein type: Oxidoreductase; EC 1.10.99.2

Chromosomal Location of Human Ortholog: 6p25.2

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: electron carrier activity; dihydronicotinamide riboside quinone reductase activity; NADPH dehydrogenase (quinone) activity; metal ion binding

Biological Process: memory

Disease: Breast Cancer