Western Blot (WB)
(Loading: 15 ug of lysates per lane. Antibodies: NPTX2 MBS150863 (A: 0.5 ug/mL, B: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at)
Rabbit NPTX2 Polyclonal Antibody | anti-NPTX2 antibody
NPTX2 Antibody
IHC: 5 ug/mL
IF: 20 ug/mL
Antibody validated: Western Blot in human, mouse and rat samples;Immunohistochemistry in human and mouse samples; Immunofluorescence in humanand mouse samples. All other applications and species not yet tested.
The immunogen is located within amino acids 170-220 of NPTX2.
Western Blot (WB)
(Loading: 15 ug of lysates per lane. Antibodies: NPTX2 MBS150863 (A: 0.5 ug/mL, B: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at)
Western Blot (WB)
(Loading: 15 ug of lysates per lane. Antibodies: NPTX2 MBS150863 (A: 0.5 ug/mL, B: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human brain using anti-NPTX2 antibody (MBS150863) at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human brain using anti-NPTX2 antibody (MBS150863) at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
Immunofluorescence (IF)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling NPTX2 with MBS150863 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).)
Immunofluorescence (IF)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling NPTX2 with MBS150863 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse brain issue using anti-NPTX2 antibody (MBS150863) at 5ug/ml. Tissue was fixed with formaldehyde and blockedwith 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse brain issue using anti-NPTX2 antibody (MBS150863) at 5ug/ml. Tissue was fixed with formaldehyde and blockedwith 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
Western Blot (WB)
(Western blot analysis of NPTX2 expression in mouse brain tissue lysate with NPTX2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Western Blot (WB)
(Western blot analysis of NPTX2 expression in mouse brain tissue lysate with NPTX2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Immunofluorescence (IF)
(Immunofluorescence of NPTX2 in mouse brain tissue with NPTX2 antibody at 20 μg/mL.Red: NPTX2 Antibody (4573)Blue: DAPI staining)
Immunofluorescence (IF)
(Immunofluorescence of NPTX2 in mouse brain tissue with NPTX2 antibody at 20 μg/mL.Red: NPTX2 Antibody (4573)Blue: DAPI staining)
Testing Data
(Loading: 15 ug of lysates per lane. Antibodies: NPTX2 MBS150863(2 ug/mL) and beta-actin MBS150383 (1ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
Testing Data
(Loading: 15 ug of lysates per lane. Antibodies: NPTX2 MBS150863(2 ug/mL) and beta-actin MBS150383 (1ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
Western Blot (WB)
(Loading: 15 ug of lysates per lane. Antibodies: NPTX2 MBS150863(1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. A: Absence of blocking peptide B: Presence of blocking peptide.)
Western Blot (WB)
(Loading: 15 ug of lysates per lane. Antibodies: NPTX2 MBS150863(1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. A: Absence of blocking peptide B: Presence of blocking peptide.)
Immunofluorescence (IF)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain issue labeling NPTX2 with MBS150863 at 20ug/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (red) and DAPI staining (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain issue labeling NPTX2 with MBS150863 at 20ug/mL, followed by goat anti-rabbit IgG secondaryantibody at 1/500 dilution (red) and DAPI staining (blue).)
NCBI and Uniprot Product Information
Observed MW: 47 KD
NCBI Description
This gene encodes a member of the family of neuronal petraxins, synaptic proteins that are related to C-reactive protein. This protein is involved in excitatory synapse formation. It also plays a role in clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. Up-regulation of this gene in Parkinson disease (PD) tissues suggests that the protein may be involved in the pathology of PD. [provided by RefSeq, Feb 2009]
Uniprot Description
NPTX2: Likely to play role in the modification of cellular properties that underlie long-term plasticity. Binds to agar matrix in a calcium-dependent manner.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 7q21.3-q22.1
Cellular Component: extracellular region
Molecular Function: metal ion binding; carbohydrate binding
Biological Process: synaptic transmission
Research Articles on NPTX2
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Product Notes
The NPTX2 nptx2 (Catalog #AAA150863) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NPTX2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NPTX2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC-P), Immunofluorescence (IF). WB: 0.5-4 ug/mL IHC: 5 ug/mL IF: 20 ug/mL Antibody validated: Western Blot in human, mouse and rat samples;Immunohistochemistry in human and mouse samples; Immunofluorescence in humanand mouse samples. All other applications and species not yet tested. Researchers should empirically determine the suitability of the NPTX2 nptx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NPTX2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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