Basonuclin 1 Recombinant Protein | BNC1 recombinant protein

Recombinant Basonuclin 1 (BNC1)

Cat. Num. AAA2033174

• Gene Names: BNC1; BNC; BSN1; HsT19447
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Basonuclin 1; Recombinant Basonuclin 1 (BNC1); BNC; BSN1; Zinc Finger Protein Basonuclin-1; BNC1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: CQSFKPGKINHRQCDQCK HGWVAHALSK LRIPPMYPTS QVEIVQSNVV FDISSLMLYGTQAIPVRLKI LLDRLFSVLK QDEVLQILHA LDWTLQDYIR GYVLQDASGKVLDHWSIMTS EEEVATLQQF LRFGETKSIV ELMAIQEKEE QSIIIPPSTANVDIRAFIES CSHRSSSLPT PVDKGNPSSI HPFENLISNM TFMLPF
• Sequence Length: 862

• Applicable Applications for BNC1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 26.9kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Cys43~Phe246
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119582828
• NCBI GeneID: 646
• Molecular Weight: 110,972 Da
• NCBI Official Full Name: basonuclin 1, partial
• NCBI Official Synonym Full Names: basonuclin 1
• NCBI Official Symbol: BNC1
• NCBI Official Synonym Symbols: BNC; BSN1; HsT19447
• NCBI Protein Information: zinc finger protein basonuclin-1
• UniProt Protein Name: Zinc finger protein basonuclin-1
• Protein Family: Cruciferin
• UniProt Gene Name: BNC1
• UniProt Synonym Gene Names: BNC
• UniProt Entry Name: BNC1_HUMAN

NCBI Description

The protein encoded by this gene is a zinc finger protein present in the basal cell layer of the epidermis and in hair follicles. It is also found in abundance in the germ cells of testis and ovary. This protein is thought to play a regulatory role in keratinocyte proliferation and it may also be a regulator for rRNA transcription. Alternative splicing of this gene results in multiple transcript variants, and multiple polyadenylation sites are indicated.[provided by RefSeq, Jul 2014]

Uniprot Description

basonuclin 1: Likely to be a transcription factor specific for squamous epithelium and for the constituent keratinocytes at a stage either prior to or at the very beginning of terminal differentiation. May play a role in the differentiation of spermatozoa and oocytes.

Protein type: C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: 15q25.2

Cellular Component: cytoplasm; nucleus

Molecular Function: DNA binding; metal ion binding; transcription factor activity

Biological Process: chromosome organization and biogenesis; epidermis development; positive regulation of cell proliferation; positive regulation of epithelial cell proliferation; regulation of transcription from RNA polymerase I promoter; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; wound healing