Rabbit anti-Human NPHP3 Polyclonal Antibody | anti-NPHP3 antibody

NPHP3, NT (NPHP3, KIAA2000, Nephrocystin-3) (FITC)

Cat. Num. AAA6326251

• Gene Names: NPHP3; MKS7; NPH3; RHPD; RHPD1; SLSN3; CFAP31
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NPHP3; Polyclonal Antibody; NT (NPHP3; KIAA2000; Nephrocystin-3) (FITC); Nephrocystin-3; anti-NPHP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NPHP3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NPHP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-181 amino acids from the N-terminal region of human NPHP3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NPHP3 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the NPHP3 antibody detected the NPHP3 protein (arrow).)

Immunohistochemistry (IHC)

(NPHP3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NPHP3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-NPHP3 antibody

This gene encodes a protein containing a coiled-coil (CC) domain, a tubulin-tyrosine ligase (TTL) domain, and a tetratrico peptide repeat (TPR) domain. The encoded protein interacts with nephrocystin and may function in renal tubular development and function. Mutations in this gene are associated with nephronophthisis type 3. Multiple splice variants have been described but their full-length nature has not been determined.

Product Categories/Family for anti-NPHP3 antibody

Antibodies; KIAA; orf

References

Simpson, M.A., et al. Am. J. Kidney Dis. 53(5):790-795(2009). Tory, K., et al. Kidney Int. 75(8):839-847(2009). Bergmann, C., et al. Am. J. Hum. Genet. 82(4):959-970(2008). Hoefele, J., et al. J. Am. Soc. Nephrol. 18(10):2789-2795(2007). Leipe, D.D., et al. J. Mol. Biol. 343(1):1-28(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 34304360
• NCBI GeneID: 27031
• NCBI Accession #: NP_694972.3
• NCBI GenBank Nucleotide #: NM_153240.4
• UniProt Accession #: Q7Z494; Q5JPE3; Q5JPE6; Q68D99; Q6NVH3; Q7Z492; Q7Z493; Q8N9R2; Q8NCM5; Q96N70; Q96NK2
• Molecular Weight: 150,864 Da
• NCBI Official Full Name: nephrocystin-3
• NCBI Official Synonym Full Names: nephronophthisis 3 (adolescent)
• NCBI Official Symbol: NPHP3
• NCBI Official Synonym Symbols: MKS7; NPH3; RHPD; RHPD1; SLSN3; CFAP31
• NCBI Protein Information: nephrocystin-3; Meckel syndrome, type 7; cilia and flagella associated protein 31
• UniProt Protein Name: Nephrocystin-3
• Protein Family: Nephrocystin
• UniProt Gene Name: NPHP3
• UniProt Synonym Gene Names: KIAA2000
• UniProt Entry Name: NPHP3_HUMAN