Western Blot (WB)
(NPHP3 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the NPHP3 antibody detected the NPHP3 protein (arrow).)
Rabbit anti-Human NPHP3 Polyclonal Antibody | anti-NPHP3 antibody
NPHP3, NT (NPHP3, KIAA2000, Nephrocystin-3) (AP)
Gene Names
NPHP3; MKS7; NPH3; RHPD; RHPD1; SLSN3; CFAP31
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
NPHP3; Polyclonal Antibody; NT (NPHP3; KIAA2000; Nephrocystin-3) (AP); Nephrocystin-3; anti-NPHP3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-NPHP3 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
NPHP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-181 amino acids from the N-terminal region of human NPHP3.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(NPHP3 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the NPHP3 antibody detected the NPHP3 protein (arrow).)
Western Blot (WB)
(NPHP3 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the NPHP3 antibody detected the NPHP3 protein (arrow).)
Immunohistochemistry (IHC)
(NPHP3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NPHP3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(NPHP3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NPHP3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-NPHP3 antibody
This gene encodes a protein containing a coiled-coil (CC) domain, a tubulin-tyrosine ligase (TTL) domain, and a tetratrico peptide repeat (TPR) domain. The encoded protein interacts with nephrocystin and may function in renal tubular development and function. Mutations in this gene are associated with nephronophthisis type 3. Multiple splice variants have been described but their full-length nature has not been determined.
Product Categories/Family for anti-NPHP3 antibody
References
Simpson, M.A., et al. Am. J. Kidney Dis. 53(5):790-795(2009). Tory, K., et al. Kidney Int. 75(8):839-847(2009). Bergmann, C., et al. Am. J. Hum. Genet. 82(4):959-970(2008). Hoefele, J., et al. J. Am. Soc. Nephrol. 18(10):2789-2795(2007). Leipe, D.D., et al. J. Mol. Biol. 343(1):1-28(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
150,864 Da
NCBI Official Full Name
nephrocystin-3
NCBI Official Synonym Full Names
nephronophthisis 3 (adolescent)
NCBI Official Symbol
NPHP3
NCBI Official Synonym Symbols
MKS7; NPH3; RHPD; RHPD1; SLSN3; CFAP31
NCBI Protein Information
nephrocystin-3; Meckel syndrome, type 7; cilia and flagella associated protein 31
UniProt Protein Name
Nephrocystin-3
Protein Family
UniProt Gene Name
NPHP3
UniProt Synonym Gene Names
KIAA2000
UniProt Entry Name
NPHP3_HUMAN
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Product Notes
The NPHP3 nphp3 (Catalog #AAA6326248) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NPHP3, NT (NPHP3, KIAA2000, Nephrocystin-3) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NPHP3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NPHP3 nphp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NPHP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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