Western Blot (WB)
(Anti-NOXA1 antibody, MBS176147, Western blottingLane 1: U87 Cell LysateLane 2: HELA Cell Lysate )
Rabbit anti-Human NOXA1 Polyclonal Antibody | anti-NOXA1 antibody
Anti-NOXA1 antibody
Western Blot (WB)
(Anti-NOXA1 antibody, MBS176147, Western blottingLane 1: U87 Cell LysateLane 2: HELA Cell Lysate )
Western Blot (WB)
(Anti-NOXA1 antibody, MBS176147, Western blottingLane 1: U87 Cell LysateLane 2: HELA Cell Lysate )
Background: NOXA1(NADPH oxidase activator 1), also called NOX ACTIVATOR 1 or p51-NOX, is an enzyme that in humans is encoded by the NOXA1 gene. Hartz(2007) mapped the NOXA1 gene to chromosome 9q34.3 based on an alignment of the NOXA1 sequence with the genomic sequence(build 36.1). Banfi et al.(2003) mapped the mouse Noxa1 gene to chromosome 2. Using yeast 2-hybrid assays, Takeya et al.(2003) showed that human p51-NOX interacted with constitutively active forms of RAC1 and RAC2. In vitro binding assays revealed that p51-NOX bound GTP-bound RAC1, but not GDP-bound RAC1. p51-NOX also bound p47-PHOX(NCF1) and p41-NOX(NOXO1), and trp436 within the SH3 domain of p51-NOX was required for these interactions. Human cell lines or COS-7 cells cotransfected with p51-NOX and p41-NOX and either gp91-PHOX(CYBB) or NOX1 produced superoxide. Cells individually transfected with NOX1, p41-NOX, or p51-NOX and cells transfected with only p41-NOX and p51-NOX showed no superoxide production.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein which activates NADPH oxidases, enzymes which catalyze a reaction generating reactive oxygen species. The encoded protein contains four N-terminal tetratricopeptide domains and a C-terminal Src homology 3 domain. Interaction between the encoded protein and proteins in the oxidase regulatory complex occur via the tetratricopeptide domains. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
Uniprot Description
NOXA1: Functions as an activator of NOX1, a superoxide- producing NADPH oxidase. Functions in the production of reactive oxygen species (ROS) which participate in a variety of biological processes including host defense, hormone biosynthesis, oxygen sensing and signal transduction. May also activate CYBB/gp91phox and NOX3. Belongs to the NCF2/NOXA1 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Activator
Chromosomal Location of Human Ortholog: 9q34.3
Cellular Component: cytoplasm; NADPH oxidase complex
Molecular Function: protein binding; enzyme binding; Rac GTPase binding; superoxide-generating NADPH oxidase activator activity; SH3 domain binding
Biological Process: positive regulation of catalytic activity; superoxide metabolic process; regulation of hydrogen peroxide metabolic process
Research Articles on NOXA1
Similar Products
Product Notes
The NOXA1 noxa1 (Catalog #AAA176147) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NOXA1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NOXA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the NOXA1 noxa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOXA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
