Rabbit anti-Mouse SHQ1 Polyclonal Antibody | anti-SHQ1 antibody

SHQ1, NT (SHQ1, Protein SHQ1 homolog) (PE)

Cat. Num. AAA6347493

• Gene Names: SHQ1; Shq1p
• Reactivity: Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SHQ1; Polyclonal Antibody; NT (SHQ1; Protein SHQ1 homolog) (PE); Protein SHQ1 homolog; anti-SHQ1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SHQ1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SHQ1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-94 amino acids from the N-terminal region of human SHQ1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SHQ1 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the SHQ1 antibody detected the SHQ1 protein (arrow).)

Immunohistochemistry (IHC)

(SHQ1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SHQ1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-SHQ1 antibody

Antibodies; Proteins

References

Grozdanov, P.N., et al. RNA 15(6):1188-1197(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 56550033
• NCBI GeneID: 55164
• NCBI Accession #: NP_060600.2
• NCBI GenBank Nucleotide #: NM_018130.2
• UniProt Accession #: Q6PI26; Q6MZJ4; Q7Z748; Q9H7E5; Q9NVS8; B4DL05
• Molecular Weight: 61,548 Da
• NCBI Official Full Name: protein SHQ1 homolog
• NCBI Official Synonym Full Names: SHQ1, H/ACA ribonucleoprotein assembly factor
• NCBI Official Symbol: SHQ1
• NCBI Official Synonym Symbols: Shq1p
• NCBI Protein Information: protein SHQ1 homolog
• UniProt Protein Name: Protein SHQ1 homolog
• Protein Family: Protein
• UniProt Gene Name: SHQ1
• UniProt Entry Name: SHQ1_HUMAN

NCBI Description

SHQ1 assists in the assembly of H/ACA-box ribonucleoproteins that function in the processing of ribosomal RNAs, modification of spliceosomal small nuclear RNAs, and stabilization of telomerase (see MIM 602322) (Grozdanov et al., 2009 [PubMed 19383767]).[supplied by OMIM, Dec 2010]

Uniprot Description

SHQ1: Required for the quantitative accumulation of H/ACA ribonucleoproteins (RNPs), including telomerase, probably through the stabilization of DKC1, from the time of its synthesis until its association with NOP10, NHP2, and NAF1 at the nascent H/ACA RNA. Belongs to the SHQ1 family.

Chromosomal Location of Human Ortholog: 3p13

Cellular Component: nucleoplasm; Cajal body; cytoplasm; nucleolus; cytosol

Molecular Function: protein binding

Biological Process: positive regulation of apoptosis; protein-RNA complex assembly