Rabbit anti-Human, Rat NOXA Polyclonal Antibody | anti-NOXA antibody

RABBIT ANTI NOXA

Cat. Num. AAA221324

• Gene Names: Pmaip1; Noxa
• Reactivity: Human, Rat
• Applications: Immunohistochemistry, Western Blot
• Synonyms: NOXA; Polyclonal Antibody; RABBIT ANTI NOXA; anti-NOXA antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises murine Noxa, an 11kDa member of the BH3-only proapoptotic protein family. Noxa is localised to the mitochondria and is a transcriptional target of p73, p53 and BH3-only proteins. It is thought to induce apoptosis when activated, lowering the activity of anti-apoptotic Bcl-2-like proteins. It can be upregulated by mitogenic activation, independently of p53. In humans, along with Mcl-1 it is thought to form a selective pathway that restrains lymphocyte proliferation.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)
• Sequence Length: 103

• Applicable Applications for anti-NOXA antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 11kDa in human stomach cell lysates.; Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide corresponding to N-terminus of mouse NOXA.; Buffer Solution
• Antiserum Preparation: Antisera to NOXA were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of human stomach tissue lysate probed with Rabbit anti NOXA (MBS221324) at 0.5(A), 1(B) and 2(C) ug/ml)

Testing Data #2

(Formalin fixed, paraffin embedded human stomach stained with Rabbit anti Human NOXA (MBS221324) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Related Product Information for anti-NOXA antibody

MBS221324 recognises murine Noxa, an 11kDa member of the BH3-only proapoptotic protein family. Noxa is localised to the mitochondria and is a transcriptional target of p73, p53 and BH3-only proteins. It is thought to induce apoptosis when activated, lowering the activity of anti-apoptotic Bcl-2-like proteins. It can be upregulated by mitogenic activation, independently of p53. In humans, along with Mcl-1 it is thought to form a selective pathway that restrains lymphocyte proliferation.

NCBI and Uniprot Product Information

• NCBI GI #: 10946832
• NCBI GeneID: 58801
• NCBI Accession #: NP_067426.1
• NCBI GenBank Nucleotide #: NM_021451.2
• UniProt Accession #: Q9JM54
• Molecular Weight: 11,566 Da
• NCBI Official Full Name: phorbol-12-myristate-13-acetate-induced protein 1
• NCBI Official Synonym Full Names: phorbol-12-myristate-13-acetate-induced protein 1
• NCBI Official Symbol: Pmaip1
• NCBI Official Synonym Symbols: Noxa
• NCBI Protein Information: phorbol-12-myristate-13-acetate-induced protein 1; protein Noxa
• UniProt Protein Name: Phorbol-12-myristate-13-acetate-induced protein 1
• Protein Family: Superoxide-generating NADPH oxidase
• UniProt Gene Name: Pmaip1
• UniProt Synonym Gene Names: Noxa
• UniProt Entry Name: APR_MOUSE

Uniprot Description

NOXA: Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1. Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1. Interacts with MCL1, BCL2A1 and BAX. Up-regulated by p53/TP53, phorbol esters, double- stranded RNA, IFNB1/IFN-beta and viruses. Highly expressed in adult T-cell leukemia cell line. Belongs to the PMAIP1 family.

Protein type: Apoptosis; Mitochondrial

Cellular Component: mitochondrion; intracellular

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of apoptosis; apoptosis; regulation of apoptosis; DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of fibroblast proliferation; positive regulation of neuron apoptosis; T cell homeostasis; positive regulation of DNA damage response, signal transduction by p53 class mediator; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to DNA damage stimulus; response to X-ray; response to UV