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Immunohistochemistry (IHC) (Anti-NOX1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Goat anti-Human NOX1 Polyclonal Antibody | anti-NOX1 antibody

Anti-NOX1 Antibody (aa490-502)

Gene Names
NOX1; MOX1; NOH1; NOH-1; GP91-2
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Immunoaffinity Purified
Synonyms
NOX1; Polyclonal Antibody; Anti-NOX1 Antibody (aa490-502); Rabbit Polyclonal to Human NOX1; MOX1; MOX-1; NADPH oxidase 1 variant NOH-1L; NADPH oxidase homolog-1; NOH-1; NOH1; Mitogenic oxidase 1; NADPH oxidase 1; gp91-2; NOX-1; anti-NOX1 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Specificity
Human NOX1. This antibody is expected to recognize both reported isoforms (NP_008983.2; NP_39249.1).
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Sequence Length
564
Applicable Applications for anti-NOX1 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
ELISA: 1:16000
IHC-P: 5 ug/ml
WB: 1-3 ug/ml
Immunogen
Synthetic peptide C-DKATDIVTGLKQK from an internal region of human NOX1 (NP_008983.2; NP_39249.1). Percent identity by BLAST analysis: Human, Gorilla, Galago (100%); Gibbon, Monkey, Marmoset, Dog, Horse, Porcine, Guinea pig (92%); Mouse, Sheep, Bovine, Hamster, Panda, Zebrafish (85%).
Conjugation
Unconjugated
Epitope
aa490-502
Preparation and Storage
Store at -20 degree C.
Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-NOX1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC) (Anti-NOX1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(NOX1 antibody (1 ug/ml) staining of MASM lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. CDK4 served as a loading control. Data provided by Everest Grant winner Dr. B. Lasseque, Emory University, Atlanta, USA)

Western Blot (WB) (NOX1 antibody (1 ug/ml) staining of MASM lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. CDK4 served as a loading control. Data provided by Everest Grant winner Dr. B. Lasseque, Emory University, Atlanta, USA)
Related Product Information for anti-NOX1 antibody
NOH-1S is a voltage-gated proton channel that mediates the H+ currentsof resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridinenucleotide-dependent oxidoreductase that generates superoxide and mightconduct H+ ions as part of its electron transport mechanism, whereasNOH-1S does not contain an electron transport chain.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
58,972 Da
NCBI Official Full Name
NADPH oxidase 1 isoform 1
NCBI Official Synonym Full Names
NADPH oxidase 1
NCBI Official Symbol
NOX1
NCBI Official Synonym Symbols
MOX1; NOH1; NOH-1; GP91-2
NCBI Protein Information
NADPH oxidase 1
UniProt Protein Name
NADPH oxidase 1
Protein Family
UniProt Gene Name
NOX1
UniProt Synonym Gene Names
MOX1; NOH1; NOX-1; MOX-1

NCBI Description

This gene encodes a member of the NADPH oxidase family of enzymes responsible for the catalytic one-electron transfer of oxygen to generate superoxide or hydrogen peroxide. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]

Uniprot Description

NOH-1S is a voltage-gated proton channel that mediates the H+ currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H+ ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain.

Research Articles on NOX1

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Product Notes

The NOX1 nox1 (Catalog #AAA2401025) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Anti-NOX1 Antibody (aa490-502) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NOX1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). ELISA: 1:16000 IHC-P: 5 ug/ml WB: 1-3 ug/ml. Researchers should empirically determine the suitability of the NOX1 nox1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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