Rabbit anti-Human MED13 Polyclonal Antibody | anti-MED13 antibody

MED13 Antibody

Cat. Num. AAA9406054

• Gene Names: MED13; ARC250; THRAP1; DRIP250; HSPC221; TRAP240
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: MED13; Polyclonal Antibody; MED13 Antibody; ARC250; Activator-recruited cofactor 250 kDa component; KIAA0593; T240; THRAP1; anti-MED13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total MED13 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 2174

• Applicable Applications for anti-MED13 antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from internal of human MED13.
• Target Name: MED13
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using MED13 antibody.)

Related Product Information for anti-MED13 antibody

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.Olsen J.V., Cell 127:635-648(2006).Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).Naeaer A.M., Nature 398:828-832(1999).

Product Categories/Family for anti-MED13 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 102468717
• NCBI GeneID: 9969
• NCBI Accession #: NP_005112.2
• NCBI GenBank Nucleotide #: NM_005121.2
• UniProt Accession #: Q9UHV7; O60334; B2RU05
• Molecular Weight: 239,297 Da
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 13
• NCBI Official Synonym Full Names: mediator complex subunit 13
• NCBI Official Symbol: MED13
• NCBI Official Synonym Symbols: ARC250; THRAP1; DRIP250; HSPC221; TRAP240
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 13
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 13
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED13
• UniProt Synonym Gene Names: ARC250; KIAA0593; THRAP1; TRAP240; ARC250; Trap240; DRIP250
• UniProt Entry Name: MED13_HUMAN

NCBI Description

This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator. [provided by RefSeq, Jul 2008]

Uniprot Description

Trap240: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 13 family.

Protein type: Nuclear receptor

Chromosomal Location of Human Ortholog: 17q22-q23

Cellular Component: nucleoplasm; membrane; Srb-mediator complex; nucleus

Molecular Function: ligand-dependent nuclear receptor transcription coactivator activity; vitamin D receptor binding; transcription coactivator activity; transcription cofactor activity; receptor activity; thyroid hormone receptor binding

Biological Process: steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter