Rabbit anti-Rat Norepinephrine Transporter Polyclonal Antibody | anti-SLC6A2 antibody

Norepinephrine Transporter (NET)

Cat. Num. AAA616472

• Gene Names: SLC6A2; NET; NAT1; NET1; SLC6A5
• Reactivity: Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Norepinephrine Transporter; Polyclonal Antibody; Norepinephrine Transporter (NET); Anti -Norepinephrine Transporter (NET); anti-SLC6A2 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat NET. Species sequence homology: mouse-100%, human, monkey and bovine-90%, chicken-85%.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA, 0.05% sodium azide with 40% glycerol.

• Applicable Applications for anti-SLC6A2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1-10ug/ml using ECL.; ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
• Immunogen: A synthentic, 22 aa mapping to the 1st extracellular domain of rat NET (KLH coupled). The peptide shows no significant homology with other known proteins.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SLC6A2 antibody

Noradrenaline transporter or epinephrine transporter (NET or NTT1 or NAT1 or SLC6A2) gene (chromosome 16) encodes a protein of 617aa (mouse 617aa, ~94% identity), with 12-13 transmembrane domains, and cytoplasmic N and C-termini. Expression of the cDNA clone in transfected HeLa cells indicated that noradrenaline transport activity is sodium-dependent and sensitive to selective noradrenaline transport inhibitors. NET mRNA has been localized to the brain stem and adrenal. The predicted protein sequence demonstrated significant amino acid identity with the Na(+)/gamma-aminobutyric acid transporter, thus identifying a new gene family for neurotransmitter transport proteins. Alternative splicing of NET mRNA produces two transporter isoforms, rNETa and rNETb, which differ at their COOH termini. The rNETa isoform reveals a COOH terminus homologous to human NET and transports norepinephrine. In contrast, rNETb revealed no detectable transport function, but reduced functional expression of rNETa when both isoforms were expressed in the same cell. Defects in NET are a cause of orthostatic intolerance (OI), a syndrome characterized by lightheadedness, fatigue, altered mentation and syncore. It is associated with postural tachycardia.

Product Categories/Family for anti-SLC6A2 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 1143479
• NCBI GeneID: 6530
• UniProt Accession #: P23975; B2R707; Q71UR5; Q96KH8; Q9BW84; Q9UQ04; Q9Y6W9
• Molecular Weight: 69,332 Da
• NCBI Official Full Name: norepinephrine transporter
• NCBI Official Synonym Full Names: solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2
• NCBI Official Symbol: SLC6A2
• NCBI Official Synonym Symbols: NET; NAT1; NET1; SLC6A5
• NCBI Protein Information: sodium-dependent noradrenaline transporter; OTTHUMP00000164270; norepinephrine transporter; neurotransmitter transporter; solute carrier family 6 member 5
• UniProt Protein Name: Sodium-dependent noradrenaline transporter
• Protein Family: Sodium-dependent noradrenaline transporter
• UniProt Gene Name: SLC6A2
• UniProt Synonym Gene Names: NAT1; NET1; SLC6A5
• UniProt Entry Name: SC6A2_HUMAN

NCBI Description

This gene encodes a member of the sodium:neurotransmitter symporter family. This member is a multi-pass membrane protein, which is responsible for reuptake of norepinephrine into presynaptic nerve terminals and is a regulator of norepinephrine homeostasis. Mutations in this gene cause orthostatic intolerance, a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.

Uniprot Description

SLC6A2: Amine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals. Defects in SLC6A2 are a cause of orthostatic intolerance (OI). OI is a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. It is associated with postural tachycardia. Plasma norepinephrine concentration is abnormally high. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A2 subfamily.

Protein type: Membrane protein, integral; Transporter; Transporter, SLC family; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: neuron projection; cell surface; membrane; integral to plasma membrane; plasma membrane; lipid raft

Molecular Function: protein binding; beta-tubulin binding; monoamine transmembrane transporter activity; alpha-tubulin binding; norephinephrine:sodium symporter activity

Biological Process: response to drug; synaptic transmission; norepinephrine transport; transport; neurotransmitter transport; monoamine transport; response to pain; transmembrane transport

Disease: Orthostatic Intolerance