Rabbit NHP2 Polyclonal Antibody | anti-NHP2 antibody

NHP2 Antibody

Cat. Num. AAA9611422

• Gene Names: NHP2; DKCB2; NHP2P; NOLA2
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: NHP2; Polyclonal Antibody; NHP2 Antibody; DKCB2; FLJ20479; H/ACA ribonucleoprotein complex subunit 2; NHP2 like protein; NHP2 ribonucleoprotein; NHP2 ribonucleoprotein homolog (yeast) 1; NHP2 ribonucleoprotein homolog; S. cerevisiae; homolog of; NHP2_HUMAN; NHP2P; NOLA2; Nucleolar protein family A member 2 (H/ACA small nucleolar RNPs); Nucleolar protein family A member 2; snoRNP protein NHP2; anti-NHP2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NHP2 Antibody detects endogenous levels of NHP2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 90

• Applicable Applications for anti-NHP2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human NHP2.
• Tissue Specificity: Expressed in brain, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen, testis and thymus. Also expressed at lower levels in the liver.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Myeloma cells, using NHP2 Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from A375 cells, using NHP2 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-NHP2 antibody

Description: Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.

Subunit Structure: Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which contains NHP2/NOLA2, GAR1/NOLA1, NOP10/NOLA3, and DKC1/NOLA4, which is presumed to be the catalytic subunit. The complex contains a stable core formed by binding of one or two NOP10-DKC1 heterodimers to NHP2; GAR1 subsequently binds to this core via DKC1. The complex binds a box H/ACA small nucleolar RNA (snoRNA), which may target the specific site of modification within the RNA substrate. During assembly, the complex contains NAF1 instead of GAR1/NOLA1. The complex also interacts with TERC, which contains a 3'-terminal domain related to the box H/ACA snoRNAs. Specific interactions with snoRNAs or TERC are mediated by GAR1 and NHP2. Associates with NOLC1/NOPP140. H/ACA snoRNPs interact with the SMN complex, consisting of SMN1 or SMN2, GEMIN2/SIP1, DDX20/GEMIN3, and GEMIN4. This is mediated by interaction between GAR1 and SMN1 or SMN2. The SMN complex may be required for correct assembly of the H/ACA snoRNP complex. Component of the telomerase holoenzyme complex at least composed of TERT, DKC1, WRAP53/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and a telomerase RNA template component (TERC).

Similarity: Belongs to the eukaryotic ribosomal protein eL8 family.

NCBI and Uniprot Product Information

• NCBI GI #: 77812674
• NCBI GeneID: 55651
• NCBI Accession #: NP_001030005.1
• NCBI GenBank Nucleotide #: NP_001030005.1
• UniProt Accession #: Q9NX24
• Molecular Weight: Observed: 17 kDa; Predicted: 18 kDa
• NCBI Official Full Name: H/ACA ribonucleoprotein complex subunit 2 isoform b
• NCBI Official Synonym Full Names: NHP2 ribonucleoprotein
• NCBI Official Symbol: NHP2
• NCBI Official Synonym Symbols: DKCB2; NHP2P; NOLA2
• NCBI Protein Information: H/ACA ribonucleoprotein complex subunit 2
• UniProt Protein Name: H/ACA ribonucleoprotein complex subunit 2
• Protein Family: NHP2-like protein
• UniProt Gene Name: NHP2
• UniProt Synonym Gene Names: NOLA2
• UniProt Entry Name: NHP2_HUMAN

NCBI Description

This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA1 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. The four H/ACA snoRNP proteins are also components of the telomerase complex. This gene encodes a protein related to Saccharomyces cerevisiae Nhp2p. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008]

Uniprot Description

NOLA2: Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine (psi) residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. Defects in NHP2 are the cause of dyskeratosis congenita autosomal recessive type 2 (DKCB2). A rare multisystem disorder caused by defective telomere maintenance. It is characterized by progressive bone marrow failure, and the clinical triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Common but variable features include premature graying, aplastic anemia, low platelets, osteoporosis, pulmonary fibrosis, and liver fibrosis among others. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy. Belongs to the ribosomal protein L7Ae family.

Protein type: Nucleolus

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: nucleoplasm; Cajal body; small nucleolar ribonucleoprotein complex; cytoplasm; nucleolus

Molecular Function: snoRNA binding

Biological Process: rRNA pseudouridine synthesis

Disease: Dyskeratosis Congenita, Autosomal Recessive, 2; Dyskeratosis Congenita, Autosomal Recessive, 1