Goat anti-Human NONO Polyclonal Antibody | anti-NONO antibody

NONO, CT (Non-POU Domain-containing Octamer-binding Protein, NonO Protein, 54kD Nuclear RNA- and DNA-binding Protein, 55kD Nuclear Protein, DNA-binding p52/p100 Complex, 52kD Subunit, NMT55, p54(nrb), p54nrb, NRB54)

Cat. Num. AAA6013159

• Gene Names: NONO; P54; NMT55; NRB54; P54NRB
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: NONO; Polyclonal Antibody; CT (Non-POU Domain-containing Octamer-binding Protein; NonO Protein; 54kD Nuclear RNA- and DNA-binding Protein; 55kD Nuclear Protein; DNA-binding p52/p100 Complex; 52kD Subunit; NMT55; p54(nrb); p54nrb; NRB54); Anti -NONO; anti-NONO antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human NONO. Species sequence homology: canine, rat and mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-NONO antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 0.1-0.3ug/ml; ELISA: 1:16,000; Immunohistochemistry (Formalin fixed paraffin embedded): 3.75ug/ml
• Immunogen: Synthetic peptide C-NRAAPGAEFAPNK from the C-terminus of human NONO.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

(Antibody (0.1ug/ml) staining of mouse brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC)

(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC)

(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

Related Product Information for anti-NONO antibody

Non-POU-domain-containing octamer binding protein (NONO) is a member of the DBHS (drosophila behavior, human splicing) domain-containing family and is an RNA- and DNA- binding protein. NONO and other DBHS domain-containing proteins are multifunctional and are reported to be involved in transcriptional regulation, mRNA processing, and DNA non-homologous end joining (NHEJ). NONO functions as a coregulator of the androgen receptor (AR) and also regulates cAMP transcriptional activity by interacting with gene promoter elements. NONO is also involved in pre-mRNA splicing through an interaction with U5 snRNA and can stimulate DNA nonhomologous end joining (NHEJ) through the interaction with ku70/G22p and ku80/XRCC5 dimers. Alternate names for NONO include 54kD nuclear RNA- and DNA-binding protein, p54 (nrb), 55kD nuclear protein, NMT55, DNA-binding p52/p100 complex, NRB54, P54, and P54NRB.

Product Categories/Family for anti-NONO antibody

Antibodies; Abs to RNA Processing

NCBI and Uniprot Product Information

• NCBI GI #: 224028246
• NCBI GeneID: 4841
• NCBI Accession #: NP_001138881.1
• NCBI GenBank Nucleotide #: NM_001145409.1
• UniProt Accession #: Q15233; O00201; P30807; Q12786; Q9BQC5; B7Z4C2; D3DVV4; F5GYZ3
• Molecular Weight: 54,232 Da
• NCBI Official Full Name: non-POU domain-containing octamer-binding protein isoform 1
• NCBI Official Synonym Full Names: non-POU domain containing, octamer-binding
• NCBI Official Symbol: NONO
• NCBI Official Synonym Symbols: P54; NMT55; NRB54; P54NRB
• NCBI Protein Information: non-POU domain-containing octamer-binding protein; p54(nrb); 55 kDa nuclear protein; 54 kDa nuclear RNA- and DNA-binding protein; DNA-binding p52/p100 complex, 52 kDa subunit; non-POU domain-containing octamer (ATGCAAAT) binding protein
• UniProt Protein Name: Non-POU domain-containing octamer-binding protein
• Protein Family: Non-POU domain-containing octamer-binding protein
• UniProt Gene Name: NONO
• UniProt Synonym Gene Names: NRB54; NonO protein; p54nrb
• UniProt Entry Name: NONO_HUMAN

NCBI Description

This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009]

Uniprot Description

NONO: DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. Together with PSPC1, required for the formation of nuclear paraspeckles. A chromosomal aberration involving NONO may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;X)(p11.2;q13.1) with TFE3.

Protein type: RNA-binding; RNA splicing; Nucleolus; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: nucleoplasm; nuclear matrix; membrane; nucleolus; nuclear speck; paraspeckles; nucleus

Molecular Function: identical protein binding; protein binding; nucleotide binding

Biological Process: circadian rhythm; transcription, DNA-dependent; RNA splicing; regulation of circadian rhythm; mRNA processing; DNA repair; negative regulation of transcription, DNA-dependent; DNA recombination